Evaluation of mouse bioassay results in an inter-laboratory comparison for paralytic shellfish poisoning toxins

Jijuan Cao; Jiang Zheng; Bing Yu; Qiuyan Wang; Junyi Xu; Aifeng Li

doi:10.1007/s00343-011-0521-9

Your Location：

Home >

Browse articles >

Evaluation of mouse bioassay results in an inter-laboratory comparison for paralytic shellfish poisoning toxins

Updated：2023-05-16

- Evaluation of mouse bioassay results in an inter-laboratory comparison for paralytic shellfish poisoning toxins
- Journal of Oceanology and Limnology Vol. 29, Issue 4, Pages: 912-916(2011)
- Affiliations：
  
  1. Liaoning Entry-Exit Inspection and Quarantine Bureau
  2. Key Laboratory of Marine Environment and Ecology Ocean University of China Ministry of Education
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0521-9
  CLC：
- Online First：10 July 2011，
  
  Published：2011
- Accepted：
Scan QR Code
Cao, J., Zheng, J., Yu, B. et al. Evaluation of mouse bioassay results in an inter-laboratory comparison for paralytic shellfish poisoning toxins., Chin. J. Ocean. Limnol. 29, 912–916 (2011). https://doi.org/10.1007/s00343-011-0521-9
DOI：

Cao, J., Zheng, J., Yu, B. et al. Evaluation of mouse bioassay results in an inter-laboratory comparison for paralytic shellfish poisoning toxins., Chin. J. Ocean. Limnol. 29, 912–916 (2011). https://doi.org/10.1007/s00343-011-0521-9 DOI：

摘要

Abstract

An inter-laboratory comparison of the AOAC mouse bioassay for paralytic shellfish poisoning (PSP) toxicity in shellfish was carried out among 25 Chinese laboratories to examine the overall performance for PSP testing in China

and to analyze the main factors affecting the performance of this method. The toxic scallop Patinopecten yessoensis collected from coast of Bohai Sea

China

was used as a test sample in the comparison study. The results were reported and evaluated using robust statistical methods. The z scores showed that 80%

and 12% of laboratories reported satisfactory results

unsatisfactory results

and questionable results

respectively. This evaluation demonstrates that the PSP mouse bioassay is an appropriate method for screening and testing PSP toxicity in shellfish. However

it was found that the experience and skill of technicians

as well as the body weight and health status of mice being used significantly affected the accuracy of the method.

关键词

Keywords

references

AOAC International. 2000. AOAC Official Method 959.08, Paralytic shellfish poison: biological method. In: Horwitz W ed. Official Methods of Analysis of AOAC International (17th ed.). Gaithersburg, MD, USA. p.59–61.

AOAC International. 2005. Natural toxins. In: Horwitz W, Latimer Jr G W eds. Official Methods of Analysis of AOAC International (18th ed.). Gaithersburg, MD, USA. Chapter 49, p.86–104.

Berry J P, Lind O. 2010. First evidence of “paralytic shellfish toxins” and cylindrospermopsin in a Mexican freshwater system, Lago Catemaco, and apparent bioaccumulation of the toxins in “tegogolo” snails (Pomacea patula catemacensis). Toxicon, 55: 930–938.

Cestèle S, Catterall W A. 2000. Molecular mechanisms of neurotoxin action on voltage-gated sodium channels. Biochimie, 82: 883–892.

Chang F H, Anderson D M, Kulis D M, Till D G. 1997. Toxin production of Alexandrium minutum (Dinophyceae) from the Bay of Plenty, New Zealand. Toxicon, 35: 393–409.

Dell’Aversano C, Walter J A, Burton I W, Stirling D J, Fattorusso E, Quilliam M A. 2008. Isolation and structure elucidation of new and unusual saxitoxin analogues from mussels. J. Nat. Prod., 71: 1 518–1 523.

Ferreira F M B, Soler J M F, Fidalgo M L, Fernández-Vila P. 2001. PSP toxins from Aphanizomenon flos-aquae(cyanobacteria) collected in the Crestuma-Lever reservoir (Douro river, northern Portugal). Toxicon, 39: 757–761.

GB/T5009.213-2008. 2008. Determination of Paralytic Shellfish Poison in Shellfish. Country Standards of the People’s Republic of China.

ISO 13528. 2005. Statistical Method for Use in Proficiency Testing by Interlaboratory Comparisons. International Organization for Standardization.

ISO/IEC 17025. 2005. General Requirements for the Competence of Testing and Calibration Laboratories. International Standard (2nd ed.). Published in Switzerland.

Lawrence J F, Niedzwiadek B, Menard C. 2005. Quantitative determination of paralytic shellfish poisoning toxins in shellfish using prechromatographic oxidation and liquid chromatography with fluorescence detection: collaborative study. J. AOAC Int., 88: 1 714–1 732.

Lefebvre K A, Bill B D, Erickson A, Baugh K A, O’Rourke L, Costa P R, Nance S, Trainer V L. 2008. Characterization of intracellular and extracellular saxitoxin levels in both field and cultured Alexandrium spp. samples from Sequim Bay, Washington. Mar. Drugs, 6: 103–116.

Li D Z, Sun L, Chen Z L, He X Y, Lin B C. 2001. Survey of the distribution of red tide toxins (Okadaic acid and Dinophytoxin-1) in the Dalian Bay sea area of China by micellar electrokinetic capillary chromatography. Electrophoresis, 22: 3 583–3 588.

Oshima Y, Blackburn S I, Hallegraeff G M. 1993. Comparative study on paralytic shellfish toxin profiles of the dinoflagellate Gymnodinium catenatum from three different countries. Mar. Biol., 116: 471–476.

Rodríguez P, Alfonso A, Botana A M, Vieytes M R, Botana L M. 2010. Comparative analysis of pre- and postcolumn oxidation methods for detection of paralytic shellfish toxins. Toxicon, 56: 448–457.

Rourke W A, Murphy C J, Pitcher G, van de Riet J M, Burns B G, Thomas K, Quilliam M A. 2008. Rapid postcolumn methodology for determination of paralytic shellfish toxins in shellfish tissue. J. AOAC Int., 91: 589–597.

Schantz E J, Mold J D, Stanger D W, Shavel J, Riel F J, Bowden J P, Lynch J M, Wyler R S, Riegel B, Sommer H. 1957. Paralytic shellfish poison. VI. A procedure for the isolation and purification of the poison from toxic clam and mussel tissues. J. Am. Chem. Soc., 79: 5 230–5 235.

Shumway S E. 1995. Phycotoxin-related shellfish poisoning: bivalve molluscs are not the only vectors. Rev. Fish. Sci., 3: 1–31.

SN/T1773-2006. 2006. Method for the Determination of Paralytic Shellfish Poison in Shellfish for Import and Export-ELISA Method. Inspection and Quarantine Professional Standards of Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China.

Usup G, Kulis D M, Anderson D M. 1994. Growth and toxin production of the toxic dinoflagellate Pyrodinium bahamense var. compressum in laboratory cultures. Nat. Toxins, 2: 254–262.

Wiese M, D’Agostino P M, Mihali T K, Moffitt M C, Neilan B A, 2010. Neurotoxic alkaloids: saxitoxin and its analogs. Mar. Drugs, 8: 2 185–2 211.

Wong J L, Oesterlin R, Rapoport H. 1971. The structure of saxitoxin. J. Am. Chem. Soc., 93: 7 344–7 345.

Zhou M J, Li J, Luckas B, Yu R C. 1999. A recent shellfish toxin investigation in China. Mar. Pollut. Bull., 39: 331–334.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

A new simple screening method for the detection of paralytic shellfish poisoning toxins

Toxicity testing of Ostreopsis cf. ovata: mouse bioassay, cytotoxicity, and hemolysis neutralization assay

Identification of paralytic shellfish toxin-producingmicroalgae using machine learning and deep learning methods

Morphological and toxicity characteristics ofPseudo-nitzschia pungens strain PP0201-01 isolated from the East China Sea

Influence of Structural Parameters of Diversion Cone on Smooth Effect of Gas Ejection Bottom Pressure Impact

Related Author

Shuaishuai Pi

Shufeng Ye

Haomin Gao

Lei Yao

Zhenyi Jiang

Yuling Song

Lei Xi

Jinping Cheng

Related Institution

School of Environmental Science and Engineering Shanghai Jiao Tong University

Key Laboratory of Marine Integrated Monitoring and Applied Technologies of Harmful Algal Blooms Chinese State Oceanic Administration

Shanghai Municipal Oceanic Bureau

Research Center of Harmful Algae & Marine Biology, Jinan University

School of Ocean, Yantai University

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰