Impact of redox-stratification on the diversity and distribution of bacterial communities in sandy reef sediments in a microcosm

Zheng Gao; Xin Wang; Angelos K. Hannides; Francis J. Sansone; Guangyi Wang

doi:10.1007/s00343-011-0316-z

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Impact of redox-stratification on the diversity and distribution of bacterial communities in sandy reef sediments in a microcosm

Updated：2023-05-16

- Impact of redox-stratification on the diversity and distribution of bacterial communities in sandy reef sediments in a microcosm
- Journal of Oceanology and Limnology Vol. 29, Issue 6, Pages: 1209-1223(2011)
- Affiliations：
  
  1. State Key Laboratory of Crop Biology College of Life Sciences Shandong Agricultural University
  2. Department of Oceanography University of Hawaii
  3. Shenzhen Engineering Laboratory for Algal Biofuel Technology Development and Application School of Environment and Energy Peking University Shenzhen Graduate School
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0316-z
  CLC：
- Online First：25 October 2011，
  
  Published：2011
- Accepted：
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Gao, Z., Wang, X., Hannides, A.K. et al. Impact of redox-stratification on the diversity and distribution of bacterial communities in sandy reef sediments in a microcosm., Chin. J. Ocean. Limnol. 29, 1209–1223 (2011). https://doi.org/10.1007/s00343-011-0316-z
DOI：

Gao, Z., Wang, X., Hannides, A.K. et al. Impact of redox-stratification on the diversity and distribution of bacterial communities in sandy reef sediments in a microcosm., Chin. J. Ocean. Limnol. 29, 1209–1223 (2011). https://doi.org/10.1007/s00343-011-0316-z DOI：

摘要

Abstract

Relationships between microbial communities and geochemical environments are important in marine microbial ecology and biogeochemistry. Although biogeochemical redox stratification has been well documented in marine sediments

its impact on microbial communities remains largely unknown. In this study

we applied denaturing gradient gel electrophoresis (DGGE) and clone library construction to investigate the diversity and stratification of bacterial communities in redox-stratified sandy reef sediments in a microcosm. A total of 88 Operational Taxonomic Units (OTU) were identified from 16S rRNA clone libraries constructed from sandy reef sediments in a laboratory microcosm. They were members of nine phyla and three candidate divisions

including Proteobacteria (Alpha-

Beta-

Gamma-

Delta-

and Epsilonproteobacteria)

Actinobacteria

Acidobacteria

Bacteroidetes

Chloroflexi

Cyanobacteria

Firmicutes

Verrucomicrobia

Spirochaetes

and the candidate divisions WS3

SO31 and AO19. The vast majority of these phylotypes are related to clone sequences from other marine sediments

but OTUs of Epsilonproteobacteria and WS3 are reported for the first time from permeable marine sediments. Several other OTUs are potential new bacterial phylotypes because of their low similarity with reference sequences. Results from the 16S rRNA

gene clone sequence analyses suggested that bacterial communities exhibit clear stratification across large redox gradients in these sediments

with the highest diversity found in the anoxic layer (15–25 mm) and the least diversity in the suboxic layer (3–5 mm). Analysis of the nosZ

and amoA gene libraries also indicated the stratification of denitrifiers and nitrifiers

with their highest diversity being in the anoxic and oxic sediment layers

respectively. These results indicated that redox-stratification can affect the distribution of bacterial communities in sandy reef sediments.

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references

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