

FOLLOWUS
1. Institute of Oceanology Chinese Academy of Sciences
2. Graduate University of Chinese Academy of Sciences
3. National Marine Environmental Monitoring Center
4. Institute of Oceanology & Marine Fisheries
Online First:25 October 2011,
Published:2011
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Wang, J., Shao, K., Cheng, B. et al. Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)., Chin. J. Ocean. Limnol. 29, 1260–1266 (2011). https://doi.org/10.1007/s00343-011-0295-0
A filamentous clone of Polysiphonia urceolata was regenerated from segments cut from the fronds of gametophytes. Unlike wild thalli with short virgate branchlets
the clone was filamentous with few branches. Many transparent trichoblasts arose from pericentral cells during the induction culture
but these were seldom observed during normal growth. The trichoblasts were uniseriate
often colorless
and formed lobed rhizoids rapidly when they came into contact with solid substrates. In addition to morphological characteristics
the photosynthetic properties and growth conditions of the clone differed from those of the mother plant. Cross-gradient light and temperature culture experiments revealed that the most favorable conditions for culture of the filamentous clone were 22°C and 95–120 μE/(m2·s) light intensity. The photosynthetic light saturation value for filaments was approx. 100 μE/(m2·s)
which is far lower than that of wild thalli. These results could be used to develop techniques for mass cultures of P. urceolata in photobioreactors for production of seed stock or bioactive products.
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