Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection

Zhan Luo; Jiquan Zhang; Fuhua Li; Xiaojun Zhang; Chengzhang Liu; Jianhai Xiang

doi:10.1007/s00343-011-0249-6

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Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection

Updated：2023-05-16

- Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection
- Journal of Oceanology and Limnology Vol. 29, Issue 5, Pages: 942-951(2011)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0249-6
  CLC：
- Online First：08 August 2011，
  
  Published：2011
- Accepted：
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Luo, Z., Zhang, J., Li, F. et al. Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection., Chin. J. Ocean. Limnol. 29, 942–951 (2011). https://doi.org/10.1007/s00343-011-0249-6
DOI：

Luo, Z., Zhang, J., Li, F. et al. Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection., Chin. J. Ocean. Limnol. 29, 942–951 (2011). https://doi.org/10.1007/s00343-011-0249-6 DOI：

摘要

Abstract

Acting as one of the pattern recognition receptors (PRRs)

C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work

a novel C-type lectin gene (named LvLec1) was cloned from the shrimp Litopenaeus vannamei. The ORF of LvLec1 is 510 bp

encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain (CRD) at the C-terminal. LvLec1 was mainly expressed in the hepatopancreas. Real-time PCR analysis indicated that the level of LvLec1 transcripts significantly changed in the hepatopancreas after the shrimp were artificially challenged with LPS

Micrococcus lysodeikticus and white spot syndrome virus (WSSV). RNAi-based silencing of LvLec1 resulted in increases in mortality when the shrimp were challenged with WSSV

and the median lethal time was reduced compared with controls. Although there was no characteristic “EPN” (Glu-Pro-Ser) or “QPD” (Gln-Pro-Asp) motif

the recombinant LvLec1

expressed in Escherichia coli BL21 (DE3)

could also agglutinate M. lysodeikticus and Vibrio anguillarum. The agglutinating activities were calcium-dependent and could be inhibited by D-mannose

D-glucose

D-galactose and N-Acetyl-D-mannose. These results suggest that LvLec1 might be involved in the immune response against WSSV and bacterial infections and contribute to non-self recognition as a pattern recognition receptor in the innate immune system of the shrimp L. vannamei.

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references

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