Characterization and application of monoclonal antibodies against Shewanella marisflavi, a novel pathogen of Apostichopus japonicus

Qiang Li; Hongli Jing; Hua Li; Yinan Wang; Dehai Xu

doi:10.1007/s00343-011-0234-0

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Characterization and application of monoclonal antibodies against Shewanella marisflavi, a novel pathogen of Apostichopus japonicus

Updated：2023-05-16

- Characterization and application of monoclonal antibodies against Shewanella marisflavi, a novel pathogen of Apostichopus japonicus
- Journal of Oceanology and Limnology Vol. 29, Issue 5, Pages: 973-980(2011)
- Affiliations：
  
  1. Key Laboratory of Mariculture Agriculture Ministry of China Dalian Ocean University
  2. . S. Department of Agriculture Agricultural Research Service Aquatic Animal Health Research Laboratory,U
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0234-0
  CLC：
- Online First：08 August 2011，
  
  Published：2011
- Accepted：
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Li, Q., Jing, H., Li, H. et al. Characterization and application of monoclonal antibodies against Shewanella marisflavi, a novel pathogen of Apostichopus japonicus, ., Chin. J. Ocean. Limnol. 29, 973–980 (2011). https://doi.org/10.1007/s00343-011-0234-0
DOI：

Li, Q., Jing, H., Li, H. et al. Characterization and application of monoclonal antibodies against Shewanella marisflavi, a novel pathogen of Apostichopus japonicus, ., Chin. J. Ocean. Limnol. 29, 973–980 (2011). https://doi.org/10.1007/s00343-011-0234-0 DOI：

摘要

Abstract

Shewanella marisflavi strain AP629 was certified as a novel pathogen of the sea cucumber Apostichopus japonicus. In this study

four monoclonal antibodies (MAbs) (3C1

3D9

2F2

2A8) against strain AP629 were developed by immunizing Balb/C mice. 3C1 and 3D9 recognized S. marisflavi only

showing no cross reactivity to other gram-negative bacteria. However

2F2 and 2A8 showed cross reactivity to all tested bacteria. Indirect immunofluorescence

and immunogold electron microscopy

showed the binding antigens of 3C1 and 3D9 were located at the secretion on the surface of strain AP629. The binding antigens of 2F2 and 2A8 were noted on the membrane of the cells. MAbs 3C1 and 3D9 recognized the lipopolysaccharide fraction of strain AP629

and 2F2 and 2A8 recognized in western-blotting protein antigens with molecular weights of 113 and 128 kDa respectively. MAbs 3C1 and 3D9 have the potential for use in pathogen diagnosis

epidemiology and studies on the mechanism of how S. marisflavi infects A. japonicus. Immunohistochemistry with 3C1 or 3D9 identified strain AP629 in the body wall of infected A. japonicus. In the adult sea cucumbers that were infected via body wall injection

positive signals were observed at the site of skin ulceration

and at the connective tissue of the non-ulcerated body wall. In addition

some large blue-stained cells aggregated at the connective tissue colonized by large numbers of bacteria. In juveniles infected via immersion infection

positive signals were observed at the cuticle of the body wall only. Our results suggest that 3C1 and 3D9 could be used in various immunological assays to study the invasion mechanism of strain AP629 in A. japonicus

the law of bacterial colonization

proliferation in different tissues of A. japonicus

and correlation between secretion on the surface of strain AP629 and its pathogenesis to A. japonicus.

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references

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