Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry

Guangshui Na; Jia Gu; Linke Ge; Peng Zhang; Zhen Wang; Chunyang Liu; Lin Zhang

doi:10.1007/s00343-011-0225-1

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Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry

Updated：2023-05-16

- Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry
- Journal of Oceanology and Limnology Vol. 29, Issue 5, Pages: 1093-1102(2011)
- Affiliations：
  
  1. National Marine Environmental Monitoring Center
  2. School of Biology Technology Dalian Polytechnic University
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0225-1
  CLC：
- Online First：08 August 2011，
  
  Published：2011
- Accepted：
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Na, G., Gu, J., Ge, L. et al. Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry., Chin. J. Ocean. Limnol. 29, 1093–1102 (2011). https://doi.org/10.1007/s00343-011-0225-1
DOI：

Na, G., Gu, J., Ge, L. et al. Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry., Chin. J. Ocean. Limnol. 29, 1093–1102 (2011). https://doi.org/10.1007/s00343-011-0225-1 DOI：

摘要

Abstract

Among pharmaceuticals and personal care products released into the aquatic environment

antibiotics are of particular concern

because of their ubiquity and health effects. Although scientists have recently paid more attention to the threat of antibiotics to coastal ecosystems

researchers have often focused on relatively few antibiotics

because of the absence of suitable analytical methods. We have therefore developed a method for the rapid detection of 36 antibiotic residues in coastal waters

including tetracyclines (TCs)

sulfanilamides (SAs)

and quinolones (QLs). The method consists of solid-phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis

using electrospray ionization (ESI) in positive mode. The SPE was performed with Oasis HLB and Oasis MCX cartridges. Chromatographic separation on a C18 column was achieved using a binary eluent containing methanol and water with 0.1% formic acid. Typical recoveries of the analytes ranged from 67.4% to 109.3% at a fortification level of 100 ng/L. The precision of the method

calculated as relative standard deviation (RSD)

was below 14.6% for all the compounds. The limits of detection (LODs) varied from 0.45 pg to 7.97 pg. The method was applied to determine the target analytes in coastal waters of the Yellow Sea in Liaoning

China. Among the tested antibiotics

31 were found in coastal waters

with their concentrations between the LOD and 212.5 ng/L. These data indicate that this method is valid for analysis of antibiotics in coastal waters. The study first reports such a large number of antibiotics along the Yellow Sea coast of Liaoning

and should facilitate future comprehensive evaluation of antibiotics in coastal ecosystems.

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references

Batt A L, Aga D S. 2005. Simultaneous analysis of multiple classes of antibiotics by ion trap LC/MS/MS for assessing surface water and groundwater contamination. Anal. Chem., 77(9): 2 940–2 947.

Boyd G R, Reemtsma H, Grimm D A et al. 2003. Pharmaceuticals and personal care products (PPCPs) in surface and treated waters of Louisiana, USA and Ontario, Canada. Sci. Total Environ., 311: 135–149.

Carballa M, Omil F, Lema J M et al. 2004. Behavior of pharmaceuticals, cosmetics and hormones in a sewage treatment plant. Water Res., 38: 2 918–2 926.

Castiglioni S, Bagnati R, Fanelli R et al. 2006. Removal of pharmaceuticals in sewage treatment plants in Italy. Environ. Sci. Technol., 40: 357–363

Chang H, Hu J Y, Asami M, Kunikane S. 2008. Simultaneous analysis of 16 sulfonamide and trimethoprim antibiotics in environmental waters by liquid chromatographyelectrospray tandem mass spectrometry. J. Chromatogr. A, 1 190: 390–393.

Conley J M, Symes S J, Kindelberger S A et al. 2008. Rapid liquid chromatography-tandem mass spectrometry method for the determination of a broad mixture of pharmaceuticals in surface water. J. Chromatogr. A, 1 185: 206–215.

Daughton C G, Ternes T A. 1999. Pharmaceuticals and personal care products in the environment: Agents of subtle changes? Environ. Health Persp., 107: 907–938.

Dussault E B, Balakrishnan V K, Sverko E et al. 2008. Toxicity of human pharmaceuticals and personal care products to benthic invertebrates. Environ. Toxicol. Chem., 27: 425–432.

Gobel A, McArdell C S, Joss A et al. 2007. Fate of sulfonamides, macrolides, and trimethoprim in different wastewater treatment technologies. Sci. Total Environ., 372: 361–371.

Golet E M, Alder A C, Giger W. 2002. Environmental exposure and risk assessment of fluoroquinolone antibacterial agents in wastewater and river water of the Glatt Valley watershed, Switzerland. Environ. Sci. Technol., 36: 3 645–3 651.

Gómez M J, Petrović M, Fernández-Alba A R et al. 2006. Determination of pharmaceuticals of various therapeutic classes by solid-phase extraction and liquid chromatography-tandem mass spectrometry analysis in hospital effluent wastewaters. J. Chromatogr. A, 1 114: 224–233.

Gros M, Petrovic M, Barcelo D. 2006. Development of a multi-residue analytical methodology based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for screening and trace level determination of pharmaceuticals in surface and wastewaters. Talanta, 70(4): 678–690.

Grujić S, Vasiljević T, Laušević M. 2009. Determination of multiple pharmaceutical classes in surface and ground waters by liquid chromatography-ion trap-tandem mass spectrometry. J. Chromatogr. A, 1 216: 4 989–5 000.

Hirsch R, Ternes T, Haberer K et al. 1999. Occurrence of antibiotics in the aquatic environment. Sci. Total Environ., 225: 109–118.

Kang J, Hick L A, Price W E. 2007. Using calibration approaches to compensate for remaining matrix effects n quantitative liquid chromatography/electrospray onization multistage mass spectrometric analysis of hytoestrogens in aqueous environmental samples. apid Commun. Mass Spectrom., 21(24): 4 065–4 072.

Karthikeyan K G, Meyer M T. 2006. Occurrence of antibiotics n wastewater treatment facilities in Wisconsin, USA. Sci. Total Environ., 361: 196–207.

Kasprzyk-Hordern B, Dinsdale R M, Guwy A J. 2008. The occurrence of pharmaceuticals, personal care products, endocrine disruptors and illicit drugs in surface water in South Wales, UK. Water Res., 42: 3 498–3 518.

Khetan S K, Collins T J. 2007. Human pharmaceuticals in the aquatic environment: A challenge to green chemistry. Chem. Rev., 107(6): 2 319–2 364.

Kolpin D W, Furlong E T, Meyer M T et al. 2002. Pharmaceuticals, hormones, and other organic wastewater contaminants in U. S. streams, 1999–2000: A national reconnaissance. Environ. Sci. Technol., 36: 1 202–1 211.

Kostopoulou M, Nikolaou A. 2008. Analytical problems and the need for sample preparation in the determination of pharmaceuticals and their metabolites in aqueous environmental matrices. Trends in Anal. Chem., 27(11): 1 023–1 035.

Lindberg R H, Olofsson U, Rendahl P et al. 2006. Behavior of fluoroquinolones and trimethoprim during mechanical, chemical, and active sludge treatment of sewage water and digestion of sludge. Environ. Sci. Technol., 40: 1 042–1 048.

Lindberg R H, Wennberg P, Johansson M I et al. 2005. Screening of human antibiotic substances and determination of weekly mass flows in five sewage treatment plants in Sweden. Environ. Sci. Technol., 39(10): 3 421–3 429.

Managaki S, Murata A, Takada H et al. 2007. Distribution of macrolides, sulfonamides, and trimethoprim in tropical waters: ubiquitous occurrence of veterinary antibiotics in the Mekong Delta. Environ. Sci. Technol., 41: 8 004–8 010.

McArdell C S, Molnar E, Suter M J F et al. 2003. Occurrence and fate of macrolide antibiotics in wastewater treatment plants and in the Glatt Valley Watershed, Switzerland. Environ. Sci. Technol., 37: 5 479–5 486.

Miao X S, Bishay F, Chen M. 2004. Occurrence of antimicrobials in the final effluents of wastewater treatment plants in Canada. Environ. Sci. Technol. 38(13): 3 533–3 541.

Mol H G J, Plaza-Bolanos P, Zomer P et al. 2008. Toward a generic extraction method for simultaneous determination of pesticides, mycotoxins, plant toxins, and veterinary drugs in feed and food matrixes. Anal. Chem., 80(24): 9 450–9 459.

Peng X Z, Wang Z D, Kuang W X et al. 2006. A preliminary study on the occurrence and behavior of sulfonamides, ofloxacin and chloramphenicol antimicrobials in wastewaters of two sewage treatment plants in Guangzhou, China. Sci. Total Environ., 371: 314–322.

Petrovic M, Gros M, Barcelo D. 2006. Multi-residue analysis of pharmaceuticals in wastewater by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. J. Chromatogr. A, 1 124: 68–81.

Ye S, Yao Z W, Na G S et al. 2007. Rapid simultaneous determination of 14 sulfonamides in wastewater by liquid chromatography tandem mass spectrometry. J. Sep. Sci., 30: 2 360–2 369.

Shao B, Chen D, Zhang J et al. 2009. Determination of 76 pharmaceutical drugs by liquid chromatography-tandem mass spectrometry in slaughterhouse wastewater. J. Chromatogr. A, 1 216: 8 312–8 318

Simmons D B D, Trudeau V L, Marlatt V L et al. 2008. Interaction of stilbene compounds with human and rainbow trout estrogen receptors. Environ. Toxicol. Chem., 27: 442–451.

Watkinson A J, Murby E J, Costanzo S D. 2007. Removal of antibiotics in conventional and advanced wastewater treatment: Implications for environmental discharge and wastewater recycling. Water Res., 41: 4 164–4 176.

Wollenberger L, Halling-Sùrensen B, Kusk K O. 2000. Acute and chronic toxicity of veterinary antibiotics to Daphnia magna. Chemosphere, 40: 723–730

Xiao Y, Chang H, Jia A et al. 2008. Trace analysis of quinolone and fluoroquinolone antibiotics from wastewaters by liquid chromatography-electrospray tandem mass spectrometry. J. Chromatogr. A, 1 214: 100–108.

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