Gene cloning and prokaryotic expression of recombinant outer membrane protein from Vibrio parahaemolyticus

Ye Yuan; Xiuli Wang; Sheping Guo; Xuemei Qiu

doi:10.1007/s00343-011-0211-7

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Gene cloning and prokaryotic expression of recombinant outer membrane protein from Vibrio parahaemolyticus

Updated：2023-05-16

- Gene cloning and prokaryotic expression of recombinant outer membrane protein from Vibrio parahaemolyticus
- Journal of Oceanology and Limnology Vol. 29, Issue 5, Pages: 952-957(2011)
- Affiliations：
  
  College of Life Sciences and Biotechnology Dalian Ocean University
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0211-7
  CLC：
- Online First：22 June 2011，
  
  Published：2011
- Accepted：
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Yuan, Y., Wang, X., Guo, S. et al. Gene cloning and prokaryotic expression of recombinant outer membrane protein from Vibrio parahaemolyticus, ., Chin. J. Ocean. Limnol. 29, 952–957 (2011). https://doi.org/10.1007/s00343-011-0211-7
DOI：

Yuan, Y., Wang, X., Guo, S. et al. Gene cloning and prokaryotic expression of recombinant outer membrane protein from Vibrio parahaemolyticus, ., Chin. J. Ocean. Limnol. 29, 952–957 (2011). https://doi.org/10.1007/s00343-011-0211-7 DOI：

摘要

Abstract

Gram-negative Vibrio parahaemolyticus is a common pathogen in humans and marine animals. The outer membrane protein of bacteria plays an important role in the infection and pathogenicity to the host. Thus

the outer membrane proteins are an ideal target for vaccines. We amplified a complete outer membrane protein gene (ompW) from V. parahaemolyticus ATCC 17802. We then cloned and expressed the gene into Escherichia coli BL21 (DE3) cells. The gene coded for a protein that was 42.78 kDa. We purified the protein using Ni-NTA affinity chromatography and Anti-His antibody Western blotting

respectively. Our results provide a basis for future application of the OmpW protein as a vaccine candidate against infection by V. parahaemolyticus. In addition

the purified OmpW protein can be used for further functional and structural studies.

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references

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Related Author

Ye Yuan

Xiuli Wang

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Xuemei Qiu

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Related Institution

College of Life Sciences and Biotechnology Dalian Fisheries University

Key Laboratory of Hydrobiology in Liaoning Province, College of Fisheries and Life Science, Dalian Ocean University

Institute of Hydrobiology Chinese Academy of Sciences

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