In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica

Jing Wang; Quanbin Zhang; Zhongshan Zhang; Yun Hou; Hong Zhang

doi:10.1007/s00343-011-0181-9

Your Location：

Home >

Browse articles >

In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica

Updated：2023-05-16

- In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica
- Journal of Oceanology and Limnology Vol. 29, Issue 3, Pages: 679-685(2011)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0181-9
  CLC：
- Online First：15 May 2011，
  
  Published：2011
- Accepted：
Scan QR Code
Wang, J., Zhang, Q., Zhang, Z. et al. In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica, ., Chin. J. Ocean. Limnol. 29, 679–685 (2011). https://doi.org/10.1007/s00343-011-0181-9
DOI：

Wang, J., Zhang, Q., Zhang, Z. et al. In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica, ., Chin. J. Ocean. Limnol. 29, 679–685 (2011). https://doi.org/10.1007/s00343-011-0181-9 DOI：

摘要

Abstract

Fucoidan

a group of sulfated heteropolysaccharides

was extracted from Laminaria japonica

an important economic alga species in China. The anticoagulant activity of fucoidan and its derivatives (including sulfated

phosphorylated

and aminated fucoidan) was examined using in-vitro anticoagulant systems. The correlation between chemical variations within the fucoidan group and anticoagulant activity was determined. The in-vitro anticoagulant properties of fucoidan and its derivatives were determined by measuring activated partial thromboplastin time (APTT)

prothrombin time (PT)

and thrombin time (TT). The results indicate anticoagulant activity in all samples using APTT and TT assays; however

only the fucoidan derivatives affected the PT assay. Thus

the fucoidan derivatives were able to inhibit both intrinsic and extrinsic blood coagulants. Fucoidan (FPS) and its derivatives presented better anticoagulant activity than low molecular weight fucoidan (DFPS) and its derivatives

suggesting that molecular weight and proper conformation are contributing factors for anticoagulant activity of polysaccharides. Amino groups have a positive charge and can thus change the charge density of fucoidan. Accordingly

among the tested samples

aminated fucoidan (NF) was the most active reflecting the importance of charge density for anticoagulant activity. Available data obtained using in-vitro models suggest that the sulfate content

sulfate/total-sugar ratio

molecular weight

and the substituted group of fucoidan are important factors for anticoagulant activity but that the influence of sulfate

phosphate and amino groups on anticoagulant activity was different.

关键词

Keywords

references

Andersson L O, Barrowcliffe T W, Holmer E, Johnson E A, Sims G E C. 1976. Anticoagulant properties of heparin fractionated by affinity chromatography on matrix-bound antithrombin III and by gel filtration. Thrombo. Res., 9: 575–583.

Bilan M I, Usov A I. 2008. Structural analysis of fucoidans. Nat. Prod. Commun., 3: 1 639–1 648.

Bitter T, Muir H M. 1962. A modified uronic acid carbazole reaction. Anal. Biochem., 4: 330–334.

Chandia N P, Matsuhiro B. 2008. Characterization of a fucoidan from Lessonia vadosa (Phaeophyta) and its anticoagulant and elicitor properties. Int. J. Biol. Macromol., 42: 235–240.

Chevolot L, Foucault A, Chaubet F, Kervarec N, Sinquin C, Fisher A M, Boisson-Vidal C. 1999. Further data on the structure of brown seaweed fucans: relationships with anticoagulant activity. Carbohydr. Res., 319: 154–165.

Cumashi A, Ushakova N A, Preobrazhenskaya M E, D’Incecco A, Piccoli A, Totani L, Tinari N, Morozevich G E, Berman A E, Bilan M I, Usov A I, Ustyuzhanina N E, Grachev A A, Sanderson C J, Kelly M, Rabinovich G A, Iacobelli S, Nifantiev N E. 2007. A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds. Glycobio., 17: 541–552.

Dace R, McBride E, Brooks K, Gander J, Buszko M, Doctor V M. 1997. Comparison of the anticoagulant action of sulfated and phosphorylated polysaccharides. Thrombo. Res., 87: 113–121.

Denson K W, Bonnar J. 1973. The measurement of heparin: A method based on the potentiation of anti-factor Xa. Thromb. Diath. Haemorrh., 30: 471–479.

Gibbons M N. 1955. The determination of methylpentoses. Analyst., 80: 267–276.

Grauffel V, Kloareg B, Mabeau S, Durand P, Jozefonvicz J. 1989. New Natural polysaccharides with potent antithrombic activity — fucans from brown-algae. Biomaterials, 10: 363–368.

Haroun-Bouhedja F, Ellouali M, Sinquin C, Boisson-Vidal C. 2000. Relationship between sulfate groups and biological activities of fucans. Thrombo. Resh., 100: 453–459.

Kawai Y, Seno N, Anno K. 1969. A modified method for chondrosulfatase assay. Anal. Biochem., 32: 314–321.

Kuznetsova T A, Besednova N N, Mamaev A N, Momot A P, Shevchenko N M, Zvyagintseva T N. 2003. Anticoagulant activity of fucoidan from brown algae Fucus evanescens of the Okhotsk Sea. Bull. Exp. Biol. Med., 136: 471–473.

Lowry O H, Leiner K Y, Wu M, Farr A L. 1954. The quantitative histochemistry of brain. J. Bio. Chem., 207: 1–17.

Matsubara K, Matsuura Y, Bacic A, Liao M L, Hori K, Miyazawa K. 2001. Anticoagulant properties of a sulfated galactan preparation from a marine green alga, Codium cylindricum. Int. J. Biol. Macromol., 28: 395–399.

Mauray S, Sternberg C, Theveniaux J, Millet J, Sinquin C, Taponbretaudiere J, Fischer A M. 1995. Venous antithrombotic and anticoagulant activities of a fucoidan fraction. Thrombo. Haemost., 74: 1 280–1 285.

Melo F R, Pereira M S, Foguel D, Mourao P A S. 2004. Antithrombin-mediated anticoagulant activity of sulfated polysaccharides — Different mechanisms for heparin and sulfated galactans. J. Bio. Chem., 279: 20 824–20 835.

Dubois M K A G, Hamilton P A, Rebers F S. 1956. Colorimetric method for determination of sugars and related substabces. Anal. chem., 28: 350–357.

Moll S, Roberts H R. 2002. Overview of anticoagulant drugs for the future. Seminars Hematology, 39: 145–157.

Mourao P A S. 2004. Use of sulfated fucans as anticoagulant and antithrombotic agents: Future perspectives. Curr. Pharm. Des., 10: 967–981.

Nishino T, Nagumo T. 1991. The sulfate-content dependence of the anticoagulant activity of a fucan sulfate from the brown seaweed Ecklonia kurome. Carbohydr. Res., 214: 193–197.

Nishino T, Nagumo T. 1992. Anticoagulant and antithrombin activities of oversulfated fucans. Carbohydr. Res., 229: 355–362.

Pereira M S, Mulloy B, Mourao P A S. 1999. Structure and anticoagulant activity of sulfated fucans — Comparison between the regular, repetitive, and linear fucans from echinoderms with the more heterogeneous and branched polymers from brown algae. J. Bio. Chem., 274: 7 656–7 667.

Quick A J. 1940. The clinical application of the hippuric acid and the prothrombin tests. Am. J. Clin. Path., 10: 222–225.

Rosenber R, Damus P S. 1973. Purification and mechanism of action of human antithrombin-heparin cofactor. J. Bio. Chem., 248: 6 490–6 505.

Rozkin M Y, Levina M N, Kameneva N S, Usov A I, Yefimov V S. 1989. Investigation of the mechanism of the anticoagulant action of fucoidans. Farmakol. itoksik., 3: 48–51.

Soeda S, Ohmagari Y, Shimeno H, Nagamatsu A. 1994. Preparation of aminated fucoidan and its evaluation as an antithrombotic and antilipemic agent. Biol. Pharm. Bull., 17: 784–788.

Visentin G P. 1999. Heparin-induced thrombocytopenia: Molecular pathogenesis. Thrombosis Haemostasis, 82: 448–456.

Wang J, Wang F, Zhang Q B, Zhang Z S, Shi X L, Li P C. 2009a. Synthesized different derivatives of low molecular fucoidan extracted from Laminaria japonica and their potential antioxidant activity in vitro. Int. J. Biol. Macromol., 44: 379–384.

Wang J, Zhang Q, Zhang Z, Li Z. 2008. Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica. Int. J. Biol. Macromol., 42: 127–132.

Wang J, Zhang Q, Zhang Z, Zhang J, Li P. 2009b. Synthesized phosphorylated and aminated derivatives of fucoidan and their potential antioxidant activity in vitro. Int. J. Biol. Macromol., 44: 170–174.

Yang J H, Du Y M, Huang R H, Wan Y Y, Li T Y. 2002. Chemical modification, characterization and structure-anticoagulant activity relationships of Chinese lacquer polysaccharides. Int. J. Biol. Macromol., 31: 55–62.

Zhao T T, Zhang Q B, Qi H M, Zhang H, Niu X Z, Xu Z H, Li Z. 2006. Degradation of porphyran from Porphyra haitanensis and the antioxidant activities of the degraded porphyrans with different molecular weight. Int. J. Biol. Macromol., 38: 45–50.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity

A preliminary comparative study of the productivity of three economic seaweeds

Light demands of juvenileLaminaria japonica

A transformation model forLaminaria Japonica (Phaeophyta, Laminariales)

Effects of ultrasonic treatment on female gametophytes ofLaminaria japonica (Phaeophyta)

Related Author

Pengcheng LI

Yukun QIN

Xueqin WANG

Rongfeng LI

Huahua YU

Song LIU

Xiaolin CHEN

Yuhao SUN

Related Institution

University of Chinese Academy of Sciences

Institute of Oceanology, Chinese Academy of Sciences

Institute of Oceanology

Experimental Marine Biological Laboratory EMBL

Qufu Normal University

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰