Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis

Yunguo Liu; Junfeng Li; Naihao Ye

doi:10.1007/s00343-011-0167-7

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Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis

Updated：2023-05-16

- Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis
- Journal of Oceanology and Limnology Vol. 29, Issue 3, Pages: 609-615(2011)
- Affiliations：
  
  1. Inspection & Quarantine Technology Center Shandong Entry-Exit Inspection & Quarantine Bureau
  2. Jiangsu Key Laboratory of Marine Biotechnology Huaihai Institute of Technology
  3. College of Chemical Engineering Qingdao University of Science & Technology
  4. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0167-7
  CLC：
- Online First：15 May 2011，
  
  Published：2011
- Accepted：
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Liu, Y., Li, J. & Ye, N. Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis, ., Chin. J. Ocean. Limnol. 29, 609–615 (2011). https://doi.org/10.1007/s00343-011-0167-7
DOI：

Liu, Y., Li, J. & Ye, N. Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis, ., Chin. J. Ocean. Limnol. 29, 609–615 (2011). https://doi.org/10.1007/s00343-011-0167-7 DOI：

摘要

Abstract

The genetic diversity of wild and hatchery populations of half-smooth tongue sole Cynoglossus semilaevis

based on observation of amplified fragment length polymorphism (AFLP) was described. Two hundred individuals from four wild populations

Laizhou (LZ)

Weihai (WH)

Qingdao (QD)

Rizhao (RZ)

and one hatchery population

Mingbo (MB)

were screened using eight different AFLP primer combinations. A total of 384 loci were screened in the five studied populations. 48.4%

51.3%

50.7%

49.3% and 45.8% of these loci were polymorphic among the individuals tested in the LZ

RZ and MB populations

respectively. The number of polymorphic loci detected by single primer combinations ranged from 17 to 35. The average heterozygosity of the LZ

RZ and MB populations was 0.072

0.093

0.092

0.090 and 0.063

respectively. The WH population showed the highest genetic diversity in terms of total number of AFLP bands

total number of polymorphic bands

average heterozygosity and percentage of low frequency (0–0.2) polymorphic loci among all the populations

while the LZ population was the lowest among the wild populations. Compared with the wild populations

the hatchery population showed a low genetic viability.

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Keywords

references

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