Phylogenetic relationships within the genus Aspidisca (Protozoa, Ciliophora, Euplotida) revealed by ITS1-5.8S-ITS2 region sequences

Jie Huang; Zhenzhen Yi; Miao Miao; Khaled A. S. Al-Rasheid; Weibo Song

doi:10.1007/s00343-011-0127-2

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Phylogenetic relationships within the genus Aspidisca (Protozoa, Ciliophora, Euplotida) revealed by ITS1-5.8S-ITS2 region sequences

Updated：2023-05-16

- Phylogenetic relationships within the genus Aspidisca (Protozoa, Ciliophora, Euplotida) revealed by ITS1-5.8S-ITS2 region sequences
- Journal of Oceanology and Limnology Vol. 29, Issue 2, Pages: 277-283(2011)
- Affiliations：
  
  1. Laboratory of Protozoology Institute of Evolution and Marine Biodiversity Ocean University of China
  2. Laboratory of Protozoology Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education South China Normal University
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0127-2
  CLC：
- Online First：02 March 2011，
  
  Published：2011
- Accepted：
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Huang, J., Yi, Z., Miao, M. et al. Phylogenetic relationships within the genus Aspidisca (Protozoa, Ciliophora, Euplotida) revealed by ITS1-5.8S-ITS2 region sequences., Chin. J. Ocean. Limnol. 29, 277–283 (2011). https://doi.org/10.1007/s00343-011-0127-2
DOI：

Huang, J., Yi, Z., Miao, M. et al. Phylogenetic relationships within the genus Aspidisca (Protozoa, Ciliophora, Euplotida) revealed by ITS1-5.8S-ITS2 region sequences., Chin. J. Ocean. Limnol. 29, 277–283 (2011). https://doi.org/10.1007/s00343-011-0127-2 DOI：

摘要

Abstract

The internal transcribed spacer regions (ITS1 and ITS2) and 5.8S rRNA genes were sequenced in six populations of four Aspidisca species

namely A. leptaspis

A. orthopogon

A. magna and A. aculeata. Phylogenetic trees were constructed by means of Bayesian inference (BI)

Maximum Parsimony (MP)

Neighbor-Joining (NJ)

and Maximum Likelihood (ML) to assess the inter- and intra-species relationships within the genus Aspidisca. All trees show similar topologies with stable supports and indicate that: (1) four well known groups

i.e.

Oligotrichia

Stichotrichia

Choreotrichia and Hypotrichia

are distinctly outlined within the class Spirotrichea

and all are monophyletic other than Hypotrichia; (2) members of Aspidisca can be distinguished well

based on the ITS1-5.8S-ITS2 region sequences

and A. leptaspis and A. magna shared a closer relationship than other species; (3) Aspidisca and Euplotes branch early in the subclass Hypotrichia. To compare the phylogenetic relationships based on different genes

SSU rRNA trees were also constructed with nearly the same species inclusion

which revealed different topologies of inter-species

inter-genera and inter-subclasses.

关键词

Keywords

references

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