Isolation and characterization of 45 Polymorphie microsatellite loci of turbot (Scophthalmus maximus) and cross-species amplification

Shiying Hou; Aijun Ma; Xin’an Wang; Zhihui Huang; Baogui Xue; Zhi Yang; Jiangbo Qu

doi:10.1007/s00343-011-0113-8

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Isolation and characterization of 45 Polymorphie microsatellite loci of turbot (Scophthalmus maximus) and cross-species amplification

Updated：2023-05-16

- Isolation and characterization of 45 Polymorphie microsatellite loci of turbot (Scophthalmus maximus) and cross-species amplification
- Journal of Oceanology and Limnology Vol. 29, Issue 2, Pages: 311-316(2011)
- Affiliations：
  
  1. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology
  2. Rushan Fisheries Technical Extension Station
  3. Yantian Tianyuan Aquatic Limited Corporation
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0113-8
  CLC：
- Online First：02 March 2011，
  
  Published：2011
- Accepted：
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Hou, S., Ma, A., Wang, X. et al. Isolation and characterization of 45 Polymorphie microsatellite loci of turbot (Scophthalmus maximus) and cross-species amplification., Chin. J. Ocean. Limnol. 29, 311–316 (2011). https://doi.org/10.1007/s00343-011-0113-8
DOI：

Hou, S., Ma, A., Wang, X. et al. Isolation and characterization of 45 Polymorphie microsatellite loci of turbot (Scophthalmus maximus) and cross-species amplification., Chin. J. Ocean. Limnol. 29, 311–316 (2011). https://doi.org/10.1007/s00343-011-0113-8 DOI：

摘要

Abstract

Turbot (Scophthalmus maximus) is a flatfish species commercially important for aquaculture. In this study

we generated a microsatellite-enriched genomic DNA library for Scophthalmus maximus

and then isolated and characterized 45 microsatellite loci by genotyping 30 individuals. The observed number of alleles ranged from 2 to 19 with an average of 6.24

while the effective number of alleles ranged from 1.30 to 11.11 with an average of 3.66. The expected heterozygosities varied from 0.235 to 0.925 4 and Polymorphie information content ranged from 0.2044 to 0.903 3

with an average of 0.622. Twelve loci deviated significantly from Hardy-Weinberg equilibrium

and no significant linkage disequilibrium was observed between any pair of loci after Bonferroni correction. In cross-species amplification

five flatfish species (Paralichthys lethostigma

Verasper moseri

platichthys stellatus

Hippoglossoides dubius and Cynoglossus semilaevis) showed at least one Polymorphie locus. These Polymorphie microsatellite loci should prove useful for Population analysis of turbot and other related species.

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references

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