Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3

Shiying Ren; Xiangqian Li; Jianbo Jia; Fei Liu; Tian Xiao

doi:10.1007/s00343-011-0071-1

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Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3

Updated：2023-05-16

- Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3
- Journal of Oceanology and Limnology Vol. 29, Issue 5, Pages: 917-921(2011)
- Affiliations：
  
  1. School of Life Science and Chemical Engineering Huaiyin Institute of Technology
  2. Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0071-1
  CLC：
- Online First：08 August 2011，
  
  Published：2011
- Accepted：
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Ren, S., Li, X., Jia, J. et al. Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3., Chin. J. Ocean. Limnol. 29, 917–921 (2011). https://doi.org/10.1007/s00343-011-0071-1
DOI：

Ren, S., Li, X., Jia, J. et al. Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3., Chin. J. Ocean. Limnol. 29, 917–921 (2011). https://doi.org/10.1007/s00343-011-0071-1 DOI：

摘要

Abstract

Hydantoinase is involved in the production of optically pure amino acids from racemic 5-mono-substituted hydantoins. We measured the D-hydantoinase activity in marine Halomonas sp. YSR-3 and amplified the D-hydantoinase gene by PCR. The gene was inserted into vector pGM-T and transformed into E. coli TOP10. The positive transformants with the D-hydantoinase gene were sequenced. The sequenced fragment comprises 1 510 base pairs. The D-hydantoinase gene from YSR-3 is 77% similar to that from Pseudomonas entomophila L4 by searching against the NCBI databse. The protein product of the YSR-3 D-hydantoinase gene is 75%

73%

and 70% similar to those from Pseudomonas fluorescens Pf-5

Marinomonas sp. MED121

and Burkholderia vietnamiensis G4

respectively. The difference of the D-hydantoinase gene between marine Halomonas sp. YSR-3 and other terrestrial organisms is distinct.

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Keywords

references

Bosshard P P, Santini Y, Grüter. 2000. Bacterial diversity and community composition in the chemocline of the meromictic alpine Lake Cadagno as revealed by 16S rDNA analysis. FEMS Microbiol. Ecol., 31(3): 173–182.

Hichem C, Marie-Joelle V. 2002. The polyphosphate kinase plays a negative role in the control of antibiotic production in Streptomyces lividans. Molecular Microbiology, 43(4): 919–930.

Hu X J, Li Q, Cong J Y. 2006. Co-expression of D-Hydantoinase and N-Carbamoylase in recombinant Escherichia coli. The Chinese Journal of Process Engineering, 6(6): 948–953. (in Chinese with English abstract)

Huang H H, Hu Z Y. 2000. Isolation, purification and properties of dihydropyrimidinase from Pseudomonas putida 9801. Journal of China Pharmaceutical University, 31(5): 389–392. (in Chinese with English abstract)

Huang H H, Li J, Hu Z Y. 2001. Optimization of liquid culture conditions of dihydropyrimidinase producing strain Pseudomonas putida 9801. Chinese Journal of Biochemical Pharmaceutics, 22(1): 15–17. (in Chinese with English abstract)

Ikenaka Y, Nanba H, Takahashi S. 1997. Production of D-N-carbamoyl amino acid from 5-substituted by-dantoin-using a recombinant hydantoinase derived from a strain of Pseudomonas, Agrobacterium or Bacillus. Eur. Patent 801131AI, Kanekafuchi.

Jiang N, Ren Y E, Qiang Y J, Lu D J, Sun W R. 1995. Breeding of hydantoinase overproduction strains by resistance of substrate analogue. Acta Microbiologica Sinica, 35(5): 342–345. (in Chinese with English abstract)

Kong R, Niu L X, Yuan J M. 2006. A preliminary study on the directed evolution of D-hydantoinase by error-prone PCR. Journal of Shanxi University, 29(4): 425–427. (in Chinese with English abstract)

Li Z Q, Liu J J, Hu Z Y, Wang Z H, Ming X. 2001. Cloning of D-hydantoinase gene from Pseudomonas and its expression in E. coli. Journal of China Pharmaceutical University, 32(3): 227–230. (in Chinese with English abstract)

Luo X, Feng R S, Hu Z Y. 2001. The millipore rapid screening of hydantoinase production strains. Journal of West China University of Medical Sciences, 32(3): 462–463. (in Chinese with English abstract)

Ma Y, Huang M, Huang C P. 2004. Breeding by ultraviolet ray mutation of L-methionine producing Strain. Journal of Sichuan University (Natural Science Edition), 41(4): 861–864. (in Chinese with English abstract)

Ren S Y, Wang Z F, Xiao T, Serge N. 2006. Isolation and identification of a marine polyphosphate-accumulating bacterium YSR-3. Oceanologia et Limnologia Sinica, 37(5): 437–443. (in Chinese with English abstract)

Shi Y W, Li H Q, Yuan J M. 2001. Purification and some properties of D-hydantoinase produced by Pseudomonas 2262. Acta Microbiologica Sinica, 41(5): 605–610. (in Chinese with English abstract)

Syldatk C, May O, Altenbuchner J, Mattes R, Siemann M. 1999. Microbial hydantoinases-industrial enzymes from the origin of life. Appl. Microbiol. Biotechnol., 51(3): 292–309.

Tan H L, Li W, Huang M. 2002. Screening of bacterial strains with high activity of L-5-thiomethylhy-dantoinase system. Journal of Sichuan University (Natural Science Edition), 39(C1): 11–14. (in Chinese with English abstract)

Yang L, Sun W R. 2001. The recent progress in the study of hydantoinase. Progress in Biotechnology, 21(3): 13–17. (in Chinese with English abstract)

Yang L, Wang J J, Xiang S H, Sun W R. 2002. Screening of strains producing hydantoinase and their enzyme production conditions. Industrial Microbiology, 32(3): 15–19. (in Chinese with English abstract)

Yuan J M, Feng X, Shi Y W. 2004. Construction strategy of recombinant harboring D-hydantoinase gene and expression activity of D-Hydantoinase. Journal of Shanxi University (Natural Science Edition), 27(1): 1–6. (in Chinese with English abstract)

Zhou K, Li C, Ye Y C, Deng Z D, Zhang B, Yang X L, Tan X G. 2008. Screening of bacterial strains producing Hydantoinase. Journal of Wuhan Bioengineering Institute, 4(4): 187–189. (in Chinese with English abstract)

Zobell C E. 1946. Marine Microbiology. Chronica Botanica Company, Waltham, USA. p.240–241.

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