Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform

Nan Li; Lei Zhang; Fuchao Li; Yuezhu Wang; Yongqiang Zhu; Hui Kang; Shengyue Wang; Song Qin

doi:10.1007/s00343-011-0056-0

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Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform

Updated：2023-05-16

- Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform
- Journal of Oceanology and Limnology Vol. 29, Issue 3, Pages: 505-513(2011)
- Affiliations：
  
  1. Yantai Institute of Coastal Zone Research Chinese Academy of Sciences
  2. Institute of Oceanology Chinese Academy of Sciences
  3. Graduate University of Chinese Academy of Sciences
  4. South China Sea Institute of Oceanology Chinese Academy of Sciences
  5. MOST Key Laboratory of Health and Disease Genomics Chinese National Human Genome Center at Shanghai,Shanghai
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0056-0
  CLC：
- Online First：15 May 2011，
  
  Published：2011
- Accepted：
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Li, N., Zhang, L., Li, F. et al. Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform., Chin. J. Ocean. Limnol. 29, 505–513 (2011). https://doi.org/10.1007/s00343-011-0056-0
DOI：

Li, N., Zhang, L., Li, F. et al. Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform., Chin. J. Ocean. Limnol. 29, 505–513 (2011). https://doi.org/10.1007/s00343-011-0056-0 DOI：

摘要

Abstract

In this study

the 454 pyrosequencing technology was used to analyze the DNA of the Microcystis aeruginosa symbiosis system from cyanobacterial algal blooms in Taihu Lake

China. We generated 183 228 reads with an average length of 248 bp. Running the 454 assembly algorithm over our sequences yielded 22 239 significant contigs. After excluding the M. aeruginosa sequences

we obtained 1 322 assembled contigs longer than 1 000 bp. Taxonomic analysis indicated that four kingdoms were represented in the community: Archaea (n = 9; 0.01%)

Bacteria (n = 98 921; 99.6%)

Eukaryota (n = 373; 3.7%)

and Viruses (n = 18; 0.02%). The bacterial sequences were predominantly Alphaproteobacteria (n = 41 805; 83.3%)

Betaproteobacteria (n = 5 254; 10.5%) and Gammaproteobacteria (n = 1 180; 2.4%). Gene annotations and assignment of COG (clusters of orthologous groups) functional categories indicate that a large number of the predicted genes are involved in metabolic

genetic

and environmental information processes. Our results demonstrate the extraordinary diversity of a microbial community in an ectosymbiotic system and further establish the tremendous utility of pyrosequencing.

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Keywords

references

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