Cloning and characterization of a delta-6 desaturase encoding gene from Nannochloropsis oculata

Xiaolei Ma; Jianzhong Yu; Baohua Zhu; Kehou Pan; Jin Pan; Guanpin Yang

doi:10.1007/s00343-011-0048-0

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Cloning and characterization of a delta-6 desaturase encoding gene from Nannochloropsis oculata

Updated：2023-05-16

- Cloning and characterization of a delta-6 desaturase encoding gene from Nannochloropsis oculata
- Journal of Oceanology and Limnology Vol. 29, Issue 2, Pages: 290-296(2011)
- Affiliations：
  
  1. Lab of Applied Microalgae Biology the Key Laboratory of Aquaculture Ministry of Education Ocean University of China
  2. College of Marine Life Sciences Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0048-0
  CLC：
- Online First：02 March 2011，
  
  Published：2011
- Accepted：
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Ma, X., Yu, J., Zhu, B. et al. Cloning and characterization of a delta-6 desaturase encoding gene from Nannochloropsis oculata, ., Chin. J. Ocean. Limnol. 29, 290–296 (2011). https://doi.org/10.1007/s00343-011-0048-0
DOI：

Ma, X., Yu, J., Zhu, B. et al. Cloning and characterization of a delta-6 desaturase encoding gene from Nannochloropsis oculata, ., Chin. J. Ocean. Limnol. 29, 290–296 (2011). https://doi.org/10.1007/s00343-011-0048-0 DOI：

摘要

Abstract

A gene (NANOC-D6D) encoding a desaturase that removes two hydrogen atoms from fatty acids at delta 6 position was isolated from a cDNA library of Nannochloropsis oculata (Droop) D. J. Hibberd (Eustigmatophyceae). The unicellular marine microalga N. oculata synthesizes rich long chain polyunsaturated fatty acids (LCPUFAs)

including eicosapentaenoic acid (20:5n-3

EPA). The deduced protein contains 474 amino acids that fold into 4 trans-membrane domains. The neighbor-joining phylogenetic tree indicates that NANOC-D6D is phylogenetically close to the delta-6 fatty acid desaturase of marine microalgae such as Glossomastix chrysoplasta

Thalassiosira pseudonana

and Phaeodactylum tricornutum. The gene was expressed in Saccharomyces cerevisiae INVScl to verify the substrate specificity of NANOC-D6D. Our results suggest that the recombinant NANOC-D6D simultaneously desaturates linoleic acid (LA) and α-linolenic acid (ALA).

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