Genetic identification of two species of Pleuronichthys by DNA barcoding

Hui Zhang; Yan Zhang; Tianxiang Gao; Pengfei Li; Hanxiang Xu

doi:10.1007/s00343-011-0040-8

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Genetic identification of two species of Pleuronichthys by DNA barcoding

Updated：2023-05-16

- Genetic identification of two species of Pleuronichthys by DNA barcoding
- Journal of Oceanology and Limnology Vol. 29, Issue 5, Pages: 967-972(2011)
- Affiliations：
  
  1. Fisheries College Ocean University of China
  2. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
  3. Marine Fisheries Research Institute of Zhejiang Province
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0040-8
  CLC：
- Online First：08 August 2011，
  
  Published：2011
- Accepted：
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Zhang, H., Zhang, Y., Gao, T. et al. Genetic identification of two species of Pleuronichthys by DNA barcoding., Chin. J. Ocean. Limnol. 29, 967–972 (2011). https://doi.org/10.1007/s00343-011-0040-8
DOI：

Zhang, H., Zhang, Y., Gao, T. et al. Genetic identification of two species of Pleuronichthys by DNA barcoding., Chin. J. Ocean. Limnol. 29, 967–972 (2011). https://doi.org/10.1007/s00343-011-0040-8 DOI：

摘要

Abstract

DNA barcoding is a new method for biological taxonomy

offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However

in this study

we identified both species using DNA barcoding (cytochrome c oxidase subunit I (COI))

the mtDNA control region and cytochrome b. The results reveal that: 1) intraspecific variation in the DNA barcode is much less than interspecific variation; 2) the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3) COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments.

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references

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