Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration

Fang Lü; Guangce Wang; Haochen Jin

doi:10.1007/s00343-011-0028-4

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Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration

Updated：2023-05-16

- Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration
- Journal of Oceanology and Limnology Vol. 29, Issue 2, Pages: 334-342(2011)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
  3. College of Marine Science and Engineering Tianjin University of Science and Technology
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0028-4
  CLC：
- Online First：02 March 2011，
  
  Published：2011
- Accepted：
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Lü, F., Wang, G. & Jin, H. Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration., Chin. J. Ocean. Limnol. 29, 334–342 (2011). https://doi.org/10.1007/s00343-011-0028-4
DOI：

Lü, F., Wang, G. & Jin, H. Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration., Chin. J. Ocean. Limnol. 29, 334–342 (2011). https://doi.org/10.1007/s00343-011-0028-4 DOI：

摘要

Abstract

Bryopsis hypnoides Lamouroux is a unique intertidal siphonous green alga whose extruded protoplasm can aggregate spontaneously in seawater to form numerous new cells that can develop into mature algal thalli. In this study

the photosynthetic responses during dehydration of both the thalli and protoplasts isolated from B. hypnoides were measured using a Dual-PAM (pulse amplitude modulation)-100 fluorometer. The results show that the photosynthetic rates of B. hypnoides thalli were maintained for an initial period

beyond which continued desiccation resulted in reduced rates of PSI and PSII. However

the photosynthetic performances of the isolated protoplasts dehydrated in air (CO2 concentration 600–700 mg/L) showed a slight increase of Y(II) at 20% water loss

but the rates decreased thereafter with declining water content. When protoplasts were dehydrated in CO2 deficient conditions (CO2 concentration 40–80 mg/L)

the values of Y(II) declined steadily with increased dehydration without an initial rise. These results indicated that the thalli and isolated protoplasts of this alga can utilize CO2 in ambient air effectively

and the photosynthetic performances of the isolated protoplasts were significantly different from that of the thalli during dehydration. Thus the protoplasts may be an excellent system for the study of stress tolerance.

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references

Abdala-Diaz R T, Cabello-Pasini A, Pérez-Rodriguez E, Alvarez R M C, Figueroa F L. 2006. Daily and seasonal variations of optimum quantum yield and phenolic compounds in Cystoseira tamariscifolia (Phaeophyta). Mar. Biol., 148: 459–465.

Andersson M, Schubert H, Pedersén M, Snoeijs P. 2006. Different patterns of carotenoid composition and photosynthesis acclimation in two tropical red algae. Mar. Biol, 149: 653–665.

Baker N R. 1991. A possible role for Photosystem II in environmental perturbations of photosynthesis. Physiol. Plant., 81: 563–570.

Baker N R. 2008. Chlorophyll fluorescence: A probe of photosynthesis in vivo. Annu. Rev. Plant. Biol, 59: 89–113.

Beach K S, Smith C M. 1997. Ecophysiology of a tropical rhodophyte β. Recovery from emersion stresses in Ahnfeltiopsis concinna (J. Ag.) Silva et DeCew. J. Exp. Mar. Biol. Ecoi, 211: 151–167.

Beer S, Ilan M. 1998. In situ measurements of photosynthetic irradiance responses of two Red Sea sponges growing under dim light conditions. Mar. Biol, 131: 613–617.

Björk M, Weil A, Semesi S, Beer S. 1997. The photosynthetic responses of eight seagrass species from Zanzibar to increased inorganic carbon concentrations. Mar. Biol, 129: 363–366.

Bukhov N, Carpentier R. 2004. Alternative Photosystem I-driven electron transport routes: mechanisms and functions. Photosynth. Res., 82: 17–33.

Davison I R, Pearson G A. 1996. Stress tolerance in intertidal seaweeds. J. Phycol., 32: 197–211.

Dromgoole F I. 1980. Desiccation resistance of intertidal and subtidal algae. Bot. Mar., 23: 149–159.

Gao K, Aruga Y. 1987. Preliminary studies on the photosynthesis and respiration of Porphyra yezoensis under emersed condition. J. Tokyo. Univ. Fish., 47: 51–65.

Gibor A. 1965. Surviving cytoplasts in vitro. Proc. Natl. Acad. Sci. USA, 54: 1 527–1 531.

Granbom M, Pedersén M. 1999. Carbon acquisition strategies of the red alga Eucheuma denticulatum. Hydrobiologia, 398/399: 349–354.

Haglund K, Björk M, Ramazanov Z, Garcia-Reina G, Pedersén M. 1992. Role of carbonic anhydrase in photosynthesis and inorganic-carbon assimilation in the red alga Gracilaria tenuistipitata. Planta, 187: 275–281.

Hodgson L M. 1981. Photosynthesis of the red alga Gastroclonium coulteri (Rhodophyta) in response to changes in temperature, light intensity, and desiccation. J. Phycol., 17: 37–42.

Johnson W S, Gigon A, Gulmon S L, Mooney H A. 1974. Comparative photosynthetic capacities of intertidal algae under exposed and submerged conditions. Ecology, 55: 450–453.

Johnston A M, Raven J A. 1986. The analysis of photosynthesis in air and water of Ascophyllum nodosum (L.) Le Jol. Oecologia, 69: 288–295.

Kim G H, Klotchkova T A, Kang Y. 2001. Life without a cell membrane: regeneration of protoplasts from disintegrated cells of the marine green alga Bryopsis plumosa. J. Cell. Sci., 114: 2009–2014.

Kobayashi K, Kanaizuka Y. 1985. Reunification if sub-cellular fraction of Bryopsis into viable cells. Plant. Sci., 40: 129–135.

Koster K L, Reisdorph N, Ramsay J L. 2003. Changing desiccation tolerance of pea embryo protoplasts during germination. J. Exp. Bot., 54: 1 607–1 614.

Kristensen I. 1968. Surf influence on the thallus of fucoids and the rate of desiccation. Sarsia, 34: 69–82.

Lin A P, Wang G C, Yang F, Pan G H. 2009. Photosynthetic parameters of sexually different parts of Porphyra katadai var. hemiphylla (Bangiales, Rhodophyta) during dehydration and re-hydration. Planta, 229: 803–810.

Lipkin Y, Beer S, Eshel A. 1993. The ability of Porphyra linearis (Rhodophyta) to tolerate prolonged periods of desiccation. Bot. Mar., 36: 517–523.

Madsen T V, Maberly S C. 1990. A comparison of air and water as environments for photosynthesis by the intertidal alga Fucus spiralis (Phaeophyta). J. Phycol, 26: 24–30.

Menzel D. 1988. How do giant plant cell cope with injury? — The wound response in siphonous green algae. Protoplasma, 144: 73–91.

Murai M, Yoshida S. 1998. Evidence for the cell wall involvement in temporal changes in freezing tolerance of Jerusalem artichoke (Helianthus tuberosus L.) tubers during cold acclimation. Plant. Cell. Physiol, 39: 97–105.

Pak J Y, Solorzano C, Arai M, Nitta T. 1991. Two distinct steps for spontaneous generation of sub-protoplasts from a disintegrated Bryopsis cell. Plant. Physiol, 96: 819–825.

Pena E J, Zingmark R, Nietch C. 1999. Comparative photosynthesis of two species of intertidal epiphytic macroalgae on mangrove roots during submersion and emersion. J. Phycol, 35: 1 206–1 214.

Quadir A, Harrison P J, Dewreede R E. 1979. The effects of emergence and submergence on the photosynthesis and respiration of marine macrophytes. Phycologia, 18: 83–88.

Roháček K, Barták M. 1999. Technique of the modulated chlorophyll fluorescence: basic concepts, useful parameters, and some applications. Photosynthetica, 37: 339–363.

Sampath-Wiley P, Neefus C D, Jahnke L S. 2008. Seasonal effects of sun exposure and emersion on intertidal seaweed physiology: Fluctuations in antioxidant contents, photosynthetic pigments and photosynthetic efficiency in the red alga Porphyra umbilicalis Kützing (Rhodophyta, Bangiales). J. Exp. Mar. Biol. Ecol, 361: 83–91.

Schonbeck M W, Norton T A. 1979. An investigation of drought avoidance in intertidal fucoid algae. Bot. Mar., 22: 133–144.

Schreiber U. 2004. Pulse-Amplitude (PAM) fluorometry and saturation pulse method. In: Papageorgiou G C, Govindjee eds. Chlorophyll Fluorescence: A signature of Photosynthesis. Springer. Dordrecht, the Netherlands, p. 279–319

Stapel J, Manuntun R, Herninga M A. 1997. Biomass loss and nutrient redistribution in an Indonesian Thalassia hemprichii seagrass bed following seasonal low tide exposure during daylight. Mar. Ecol. Prog. Ser., 148: 251–262.

Tatewaki M, Nagata K. 1970. Surviving protoplast in vitro and their development in Bryopsis. J. Phycol., 6: 401–403.

Uemura M, Joseph R A, Steponkus P L. 1995. Cold acclimation of Arabidopsis thaliana (effect on plasma membrane lipid composition and freeze-induced lesions). Plant. Physiol, 109: 15–30.

Webb M S, Uemura M, Steponkus P L. 1994. A comparison of freezing injury in oat and rye: two cereals at the extremes of freezing tolerance. Plant. Physiol., 104: 467–478.

Xiao L, Koster K L. 2001. Desiccation tolerance of protoplasts isolated from pea embryos. J. Exp. Bot., 52:2105–2114.

Yabe T, Ikusirna I, Tsuchiya T. 1995. Production and population ecology of Phyllospadix iwatensis Makino. I. Leaf growth and biomass in an intertidal zone. Ecol. Res., 10: 291–299.

Ye N H, Wang G C, Wang F Z, Zeng C K. 2005. Formation and growth of Bryopsis hypnoides Lamouroux regenerated from its protoplasts. J. Integr. Plant. Biol., 47: 856–862.

Zacher K, Roleda M Y, Hanelt D, Wiencke C. 2007. UV effects on photosynthesis and DNA in propagules of three Antarctic seaweeds (Adenocystis utricularis, Monostroma hariotii and Porphyra endiviifolium). Planta, 225: 1505–1516.

Zou D H, Gao K S. 2002. Effects of desiccation and CO2 concentrations on emersed photosynthesis in Porphyra haitanensis (Bangiales, Rhodophyta), a species farmed in China. Eur. J. Phycol, 37: 587–592.

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