Expression of organophosphorus-degradation gene (opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria

Qiong Li; Qing Tang; Xudong Xu; Hong Gao

doi:10.1007/s00343-010-9999-9

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Expression of organophosphorus-degradation gene (opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria

Updated：2023-05-16

- Expression of organophosphorus-degradation gene (opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria
- Journal of Oceanology and Limnology Vol. 28, Issue 6, Pages: 1248-1253(2010)
- Affiliations：
  
  1. State Key Laboratory for Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan
  2. Graduate University of the Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9999-9
  CLC：
- Online First：10 November 2010，
  
  Published：2010
- Accepted：
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Li, Q., Tang, Q., Xu, X. et al. Expression of organophosphorus-degradation gene (opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria., Chin. J. Ocean. Limnol. 28, 1248–1253 (2010). https://doi.org/10.1007/s00343-010-9999-9
DOI：

Li, Q., Tang, Q., Xu, X. et al. Expression of organophosphorus-degradation gene (opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria., Chin. J. Ocean. Limnol. 28, 1248–1253 (2010). https://doi.org/10.1007/s00343-010-9999-9 DOI：

摘要

Abstract

Genetic engineering in filamentous N2-fixing cyanobacteria usually involves Anabaena sp. PCC 7120 and several other non-aggregating species. Mass culture and harvest of such species are more energy consuming relative to aggregating species. To establish a gene transfer system for aggregating species

we tested many species of Anabaena and Nostoc

and identified Nostoc muscorum FACHB244 as a species that can be genetically manipulated using the conjugative gene transfer system. To promote biodegradation of organophosphorus pollutants in aquatic environments

we introduced a plasmid containing the organophosphorus-degradation gene (opd) into Anabaena sp. PCC 7120 and Nostoc muscorum FACHB244 by conjugation. The opd gene was driven by a strong promoter

P psbA . From both species

we obtained transgenic strains having organophosphorus-degradation activities. At 25°C

the whole-cell activities of the transgenic Anabaena and Nostoc strains were 0.163±0.001 and 0.289±0.042 unit/μg Chl a

respectively. However

most colonies resulting from the gene transfer showed no activity. PCR and DNA sequencing revealed deletions or rearrangements in the plasmid in some of the colonies. Expression of the green fluorescent protein gene from the same promoter in Anabaena sp. PCC 7120 showed similar results. These results suggest that there is the potential to promote the degradation of organophosphorus pollutants with transgenic cyanobacteria and that selection of high-expression transgenic colonies is important for genetic engineering of Anabaena and Nostoc species. For the first time

we established a gene transfer and expression system in an aggregating filamentous N2-fixing cyanobacterium. The genetic manipulation system of Nostoc muscorum FACHB244 could be utilized in the elimination of pollutants and large-scale production of valuable proteins or metabolites.

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references

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