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1. Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education) College of Environmental Science and Engineering Nankai University
2. Woods Hole Oceanographic Institution
3. Research Center for Red Tide and Aquatic Environments Jinan University
Online First:10 November 2010,
Published:2010
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Tong, M., Zhou, Q., David, K.M. et al. Culture techniques and growth characteristics of Dinophysis acuminata and its prey., Chin. J. Ocean. Limnol. 28, 1230–1239 (2010). https://doi.org/10.1007/s00343-010-9960-y
The dinoflagellate Dinophysis acuminata Claparede & Lachmann is a toxic alga that causes diarrhetic shellfish poisoning. No Dinophysis species were maintained in culture for a long period of time until 2006 when Park successfully established D. acuminata in culture using a three-step feeding protocol in which the cryptophyte
Geminigera cryophila
is fed to Myrionecta rubra (=Mesodinium rubrum)
a ciliate that is in turn fed to D. acuminata. In this paper
we present the details of culturing D. acuminata from the Northeastern United States. The protocols described herein can be adopted for laboratory studies of this species. The effects of temperature on the growth and ingestion rates of D. acuminata were also examined. The results show that D. acuminata growth rate was 0.23 /d at 10°C and 0.11 /d at 4°C when fed M. rubra prey. The maximum prey ingestion rate was 2.80 Dinophysis cell/d at 10°C
although the rate decreased slightly at 4°C. In overall
temperature showed a greater influence on growth rate of D. acuminata than on the ingestion rate under the study conditions
and the quantity of available food was also an important regulator to D. acuminata growth.
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