Detection of lymphocystis disease virus in Japanese flounder Paralichthys olivaceus and other marine teleosts from northern China

Wenbin Zhan; Yongqin Li; Xiuzhen Sheng; Jing Xing; Xiaoqian Tang

doi:10.1007/s00343-010-9934-0

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Detection of lymphocystis disease virus in Japanese flounder Paralichthys olivaceus and other marine teleosts from northern China

Updated：2023-05-16

- Detection of lymphocystis disease virus in Japanese flounder Paralichthys olivaceus and other marine teleosts from northern China
- Journal of Oceanology and Limnology Vol. 28, Issue 6, Pages: 1213-1220(2010)
- Affiliations：
  
  Laboratory of Pathology and Immunology of Aquatic Animals LMMEC Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9934-0
  CLC：
- Online First：24 June 2010，
  
  Published：2010
- Accepted：
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Zhan, W., Li, Y., Sheng, X. et al. Detection of lymphocystis disease virus in Japanese flounder Paralichthys olivaceus and other marine teleosts from northern China., Chin. J. Ocean. Limnol. 28, 1213–1220 (2010). https://doi.org/10.1007/s00343-010-9934-0
DOI：

Zhan, W., Li, Y., Sheng, X. et al. Detection of lymphocystis disease virus in Japanese flounder Paralichthys olivaceus and other marine teleosts from northern China., Chin. J. Ocean. Limnol. 28, 1213–1220 (2010). https://doi.org/10.1007/s00343-010-9934-0 DOI：

摘要

Abstract

We isolated a strain of lymphocystis disease virus (LCDV) from Japanese flounder (Paralichthys olivaceus) cultured in northern China. Based on published sequences of major capsid protein (MCP) gene of LCDV-cn (GenBank: AF126405)

we designed two primer sets P1/P2 and P3/P4. We then used one-step or nested PCR and in-situ hybridization (ISH) to detect LCDV and identify the target tissues or cells in infected Japanese flounder. The PCR products were positive in purified viral supernatant

skin nodules

gut

gill

kidney

spleen

stomach

heart

and liver of Japanese flounder. We compared the DNA sequence with 14 MCP nucleotide sequences from GenBank

including Megalocytivirus (OFIV and RSIV)

Iridovirus (CzIV and WIV)

Ranavirus (TFV and FV3)

and Lymphocystivirus (8 LCDV). Based on the alignment

we confirmed the PCR product was from Lymphocystivirus (GenBank accession number DQ279090 (LCDV-HD)). Using ISH

we noted the presence of LCDV in the skin nodules

gut

gill

spleen

stomach

and heart of spontaneously infected Japanese flounders. We successfully amplified LCDV fragments from Schlegel’s black rockfish (Sebastes schlegeli Higendorf)

redwing sea robin (Lepidotrigla microptera Günther) and turbot (Scophthalmus maximus) using the one-step and nested PCR

suggesting the target genes can be widely detected in fish using this method.

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references

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