Quantitative comparison of the expression of myogenic regulatory factors in flounder (Paralichthys olivaceus) embryos and adult tissues

Yuqing Zhang; Xungang Tan; Peng Xu; Wei Sun; Yongli Xu; Peijun Zhang

doi:10.1007/s00343-010-9286-9

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Quantitative comparison of the expression of myogenic regulatory factors in flounder (Paralichthys olivaceus) embryos and adult tissues

Updated：2023-05-16

- Quantitative comparison of the expression of myogenic regulatory factors in flounder (Paralichthys olivaceus) embryos and adult tissues
- Journal of Oceanology and Limnology Vol. 28, Issue 2, Pages: 248-253(2010)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. Graduate School of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9286-9
  CLC：
- Online First：20 March 2010，
  
  Published：2010
- Accepted：
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Zhang, Y., Tan, X., Xu, P. et al. Quantitative comparison of the expression of myogenic regulatory factors in flounder (Paralichthys olivaceus) embryos and adult tissues., Chin. J. Ocean. Limnol. 28, 248–253 (2010). https://doi.org/10.1007/s00343-010-9286-9
DOI：

Zhang, Y., Tan, X., Xu, P. et al. Quantitative comparison of the expression of myogenic regulatory factors in flounder (Paralichthys olivaceus) embryos and adult tissues., Chin. J. Ocean. Limnol. 28, 248–253 (2010). https://doi.org/10.1007/s00343-010-9286-9 DOI：

摘要

Abstract

MyoD

Myf5

and myogenin are myogenic regulatory factors that play important roles during myogenesis. It is thought that MyoD and Myf5 are required for myogenic determination

while myogenin is important for terminal differentiation and lineage maintenance. To better understand the function of myogenic regulatory factors in muscle development of flounder

an important economic fish in Asia

real-time quantitative RT-PCR was used to characterize the expression patterns of MyoD

Myf5

and myogenin at early stages of embryo development

and in different tissues of the adult flounder. The results show that

Myf5 is the first gene to be expressed during the early stages of flounder development

followed by MyoD and myogenin. The expressions of Myf5

yoD

and myogenin at the early stages have a common characteristic: expression gradually increased to a peak level

and then gradually decreased to an extremely low level. In the adult flounder

the expression of the three genes in muscle is much higher than that in other tissues

indicating that they are important for muscle growth and maintenance of grown fish. During embryonic stages

the expression level of MyoD might serve an important role in the balance between muscle cell differentiation and proliferation. When the MyoD expression is over 30% of its highest level

the muscle cells enter the differentiation stage.

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Keywords

references

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