Molecular cloning and mRNA expression analysis of myosin heavy chain (MyHC) from fast skeletal muscle of grass carp, Ctenopharyngodon idella

Wuying Chu; Guihong Fu; Shiyu Bing; Tao Meng; Ruixue Zhou; Jia Cheng; Falan Zhao; Hongfang Zhang; Jianshe Zhang

doi:10.1007/s00343-010-9283-z

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Molecular cloning and mRNA expression analysis of myosin heavy chain (MyHC) from fast skeletal muscle of grass carp, Ctenopharyngodon idella

Updated：2023-05-16

- Molecular cloning and mRNA expression analysis of myosin heavy chain (MyHC) from fast skeletal muscle of grass carp, Ctenopharyngodon idella
- Journal of Oceanology and Limnology Vol. 28, Issue 2, Pages: 239-247(2010)
- Affiliations：
  
  1. Department of Bioengineering and Environmental Science Changsha University
  2. College of Life Science Guangxi Normal University
  3. College of Life Science Jiujiang University
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9283-z
  CLC：
- Online First：20 March 2010，
  
  Published：2010
- Accepted：
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Chu, W., Fu, G., Bing, S. et al. Molecular cloning and mRNA expression analysis of myosin heavy chain (MyHC) from fast skeletal muscle of grass carp, Ctenopharyngodon idella, ., Chin. J. Ocean. Limnol. 28, 239–247 (2010). https://doi.org/10.1007/s00343-010-9283-z
DOI：

Chu, W., Fu, G., Bing, S. et al. Molecular cloning and mRNA expression analysis of myosin heavy chain (MyHC) from fast skeletal muscle of grass carp, Ctenopharyngodon idella, ., Chin. J. Ocean. Limnol. 28, 239–247 (2010). https://doi.org/10.1007/s00343-010-9283-z DOI：

摘要

Abstract

The myosin heavy chain (MyHC) is one of the major structural and contracting proteins of muscle. We have isolated the cDNA clone encoding MyHC of the grass carp

Ctenopharyngodon idella. The sequence comprises 5 934 bp

including a 5 814 bp open reading frame encoding an amino acid sequence of 1 937 residues. The deduced amino acid sequence showed 69% homology to rabbit fast skeletal MyHC and 73%–76% homology to the MyHCs from the mandarin fish

walleye pollack

white croaker

chum salmon

and carp. The putative sequences of subfragment-1 and the light meromyosin region showed 61.4%–80% homology to the corresponding regions of other fish MyHCs. The tissue-specific and developmental stage-specific expressions of the MyHC gene were analyzed by quantitative real-time PCR. The MyHC gene showed the highest expression in the muscles compared with the kidney

spleen and intestine. Developmentally

there was a gradual increase in MyHC mRNA expression from the neural formation stage to the tail bud stage. The highest expression was detected in hatching larva. Our work on the MyHC gene from the grass carp has provided useful information for fish molecular biology and fish genomics.

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references

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