Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization

Baoyu Zhang; Guofu Chen; Guangce Wang; Douding Lu

doi:10.1007/s00343-010-9268-y

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Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization

Updated：2023-05-16

- Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization
- Journal of Oceanology and Limnology Vol. 28, Issue 2, Pages: 310-314(2010)
- Affiliations：
  
  1. The Institute of Oceanology Chinese Academy of Sciences
  2. Harbin Institute of Technology
  3. Tianjin University of Science and Technology
  4. The Second Institute of Oceanography SOA
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9268-y
  CLC：
- Online First：20 March 2010，
  
  Published：2010
- Accepted：
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Zhang, B., Chen, G., Wang, G. et al. Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization., Chin. J. Ocean. Limnol. 28, 310–314 (2010). https://doi.org/10.1007/s00343-010-9268-y
DOI：

Zhang, B., Chen, G., Wang, G. et al. Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization., Chin. J. Ocean. Limnol. 28, 310–314 (2010). https://doi.org/10.1007/s00343-010-9268-y DOI：

摘要

Abstract

A harmful algae bloom (HAB) is a dense aggregation of algae in a marine or aquatic environment that can result in significant environmental problems. To forecast the occurrence of HAB

development of a rapid and precise detection method is urgently required. In this study

two Skeletonema costatum-like diatoms (SK-1 and SK-2)

were identified morphologically under a light microscope

and detected using fluorescent in situ hybridization (FISH). Strain SK-1 was isolated from a frequently HAB affected area of the East China Sea

and strain SK-2 from an aquatic farm in Qingdao

China. Fluorescent DNA probes were designed that were complementary to the ITS sequence (including 5.8S rDNA) of strain SK-1. After hybridization

strong green fluorescence was observed in cells of strain SK-1 under an epifluorescence microscope; however

no such fluorescence was observed with strain SK-2

which indicates that probes hybridized only the DNA of the target strain

SK-1

in species-specific manner

and that the two strains do not belong to a same species. This finding was confirmed by ITS sequence analysis. The FISH technique used in this study was sensitive

simple

and rapid

and is a promising tool for detecting target HAB species in natural environments.

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Keywords

references

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