Distribution and expression in vitro and in vivo of DNA vaccine against lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus)

Fengrong Zheng; Xiuqin Sun; Hongzhan Liu; Xingan Wu; Nan Zhong; Bo Wang; Guodong Zhou

doi:10.1007/s00343-010-9248-2

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Distribution and expression in vitro and in vivo of DNA vaccine against lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus)

Updated：2023-05-16

- Distribution and expression in vitro and in vivo of DNA vaccine against lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus)
- Journal of Oceanology and Limnology Vol. 28, Issue 1, Pages: 67-74(2010)
- Affiliations：
  
  1. First Institute of Oceanography SOA
  2. Marine College Shandong University at Weihai
  3. Department of Microbiology Fourth Military Medical University
  4. Hainan Green Food Industrial company Ltd
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9248-2
  CLC：
- Online First：27 January 2010，
  
  Published：2010
- Accepted：
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Zheng, F., Sun, X., Liu, H. et al. Distribution and expression in vitro and in vivo of DNA vaccine against lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus)., Chin. J. Ocean. Limnol. 28, 67–74 (2010). https://doi.org/10.1007/s00343-010-9248-2
DOI：

Zheng, F., Sun, X., Liu, H. et al. Distribution and expression in vitro and in vivo of DNA vaccine against lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus)., Chin. J. Ocean. Limnol. 28, 67–74 (2010). https://doi.org/10.1007/s00343-010-9248-2 DOI：

摘要

Abstract

Lymphocystis disease

caused by the lymphocystis disease virus (LCDV)

is a significant worldwide problem in fish industry causing substantial economic losses. In this study

we aimed to develop the DNA vaccine against LCDV

using DNA vaccination technology. We evaluated plasmid pEGFP-N2-LCDV1.3 kb as a DNA vaccine candidate. The plasmid DNA was transiently expressed after liposome transfection into the eukaryotic COS 7 cell line. The distribution and expression of the DNA vaccine (pEGFP-N2-LCDV1.3kb) were also analyzed in tissues of the vaccinated Japanese flounder by PCR

RT-PCR and fluorescent microscopy. Results from PCR analysis indicated that the vaccine-containing plasmids were distributed in injected muscle

the muscle opposite the injection site

the hind intestine

gill

spleen

head

kidney and liver

6 and 25 days after vaccination. The vaccine plasmids disappeared 100 d post-vaccination. Fluorescent microscopy revealed green fluorescence in the injected muscle

the muscle opposite the injection site

the hind intestine

gill

spleen

head

kidney and liver of fish 48 h post-vaccination

green fluorescence did not appear in the control treated tissue. Green fluorescence became weak at 60 days post-vaccination. RT-PCR analysis indicated that the mcp gene was expressed in all tested tissues of vaccinated fish 6–50 days post-vaccination. These results demonstrate that the antigen encoded by the DNA vaccine is distributed and expressed in all of the tissues analyzed in the vaccinated fish. The antigen would therefore potentially initiate a specific immune response. the plasmid DNA was injected into Japanese flounder (Paralichthys olivaceus) intramuscularly and antibodies against LCDV were evaluated. The results indicate that the plasmid encoded DNA vaccine could induce an immune response to LCDV and would therefore offer immune protection against LCD. Further studies are required for the development and application of this promising DNA vaccine.

关键词

Keywords

references

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