Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals

Jianguo Liu; Xiaoli Zhang; Yanhong Sun; Wei Lin

doi:10.1007/s00343-010-9244-6

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Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals

Updated：2023-05-16

- Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals
- Journal of Oceanology and Limnology Vol. 28, Issue 1, Pages: 1-9(2010)
- Affiliations：
  
  1. Research and Development Center of Marine Biotechnology Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9244-6
  CLC：
- Online First：27 January 2010，
  
  Published：2010
- Accepted：
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Liu, J., Zhang, X., Sun, Y. et al. Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals., Chin. J. Ocean. Limnol. 28, 1–9 (2010). https://doi.org/10.1007/s00343-010-9244-6
DOI：

Liu, J., Zhang, X., Sun, Y. et al. Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals., Chin. J. Ocean. Limnol. 28, 1–9 (2010). https://doi.org/10.1007/s00343-010-9244-6 DOI：

摘要

Abstract

The antioxidative capacity of astaxanthin and enzyme activity of reactive oxygen eliminating enzymes such as superoxide dismutase (SOD)

peroxidase (POD)

catalase (CAT) and ascorbate peroxidase (APX) were studied in three cell types of Haematococcus pluvialis exposed to high concentrations of a superoxide anion radical (O 2 − ). The results show that defensive enzymes and astaxanthin-related mechanisms were both active in H. pluvialis during exposure to reactive oxygen species (ROS) such as O 2 − . Astaxanthin reacted with ROS much faster than did the protective enzymes

and had the strongest antioxidative capacity to protect against lipid peroxidation. The defensive mechanisms varied significantly between the three cell types and were related to the level of astaxanthin that had accumulated in those cells. Astaxanthin-enriched red cells had the strongest antioxidative capacity

followed by brown cells

and astaxanthin-deficient green cells. Although there was no significant increase in expression of protective enzymes

the malondialdehyde (MDA) content in red cells was sustained at a low level because of the antioxidative effect of astaxanthin

which quenched O 2 − before the protective enzymes could act. In green cells

astaxanthin is very low or absent; therefore

scavenging of ROS is inevitably reliant on antioxidative enzymes. Accordingly

in green cells

these enzymes play the leading role in scavenging ROS

and the expression of these enzymes is rapidly increased to reduce excessive ROS. However

because ROS were constantly increased in this study

the enhance enzyme activity in the green cells was not able to repair the ROS damage

leading to elevated MDA content. Of the four defensive enzymes measured in astaxanthin-deficient green cells

SOD eliminates O 2 −

POD eliminates H2O2

which is a by-product of SOD activity

and APX and CAT are then initiated to scavenge excessive ROS.

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Keywords

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