Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)

Qianru Zhou; Mingyu Shao; Zhenkui Qin; Ho Kang Kyoung; Zhifeng Zhang

doi:10.1007/s00343-010-9231-y

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Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)

Updated：2023-05-16

- Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)
- Journal of Oceanology and Limnology Vol. 28, Issue 1, Pages: 37-45(2010)
- Affiliations：
  
  1. Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of China
  2. Department of Aquaculture Chonnam National University
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9231-y
  CLC：
- Online First：27 January 2010，
  
  Published：2010
- Accepted：
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Zhou, Q., Shao, M., Qin, Z. et al. Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)., Chin. J. Ocean. Limnol. 28, 37–45 (2010). https://doi.org/10.1007/s00343-010-9231-y
DOI：

Zhou, Q., Shao, M., Qin, Z. et al. Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)., Chin. J. Ocean. Limnol. 28, 37–45 (2010). https://doi.org/10.1007/s00343-010-9231-y DOI：

摘要

Abstract

RNA helicases of the DEAD-box and related families are involved in various cellular processes including DNA replication

DNA repair

and RNA processing. However

the function of DEAD-box proteins in aquaculture species is poorly understood at molecular level. We obtained the full-length cDNA sequences of two genes encoding helicase-related proteins

Fc-vasa and Fc-PL10a

from the testes of Chinese shrimp

Fenneropenaeus chinensis. The two predicted amino acid sequences contain all the conserved motifs characterized by the DEAD-box family and several RGG repeats in the N-terminal regions. Homology and phylogenetic analyses indicate that they belong to the vasa and PL10 subfamilies. The three-dimensional structures of the two proteins were predicted with a homology modeling approach. Both core proteins consist of two tandem RecA-like domains similar to those of the DEAD-box RNA helicase. Using reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR we found that Fc-vasa was expressed specifically in the adult gonads. Transcription decreased in the ovary but increased in the testis during gonadal development. Fc-PL10a expression was widely distributed in the tissues we examined. Using in situ hybridization

we demonstrated that the Fc-vasa transcript is localized to the cytoplasm of the spermatogonia and oocytes. Thus

our results suggest that Fc-vasa plays an important role in germ-line development

and has utility as a germ cell lineage marker which will help to generate new insight into the origin and differentiation of germ cells as well as the regulation of reproduction in F. chinensis.

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references

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