Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara

Qingpi Yan; Minhui Zhao; Xiaolu Wang; Wenzheng Zou; Changsheng Chen

doi:10.1007/s00343-010-9229-5

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Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara

Updated：2023-05-16

- Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara
- Journal of Oceanology and Limnology Vol. 28, Issue 2, Pages: 260-266(2010)
- Affiliations：
  
  Key Laboratory of Science and Technology for Aquaculture and Food Safety Fisheries College of Jimei University
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9229-5
  CLC：
- Online First：20 March 2010，
  
  Published：2010
- Accepted：
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Yan, Q., Zhao, M., Wang, X. et al. Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara, ., Chin. J. Ocean. Limnol. 28, 260–266 (2010). https://doi.org/10.1007/s00343-010-9229-5
DOI：

Yan, Q., Zhao, M., Wang, X. et al. Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara, ., Chin. J. Ocean. Limnol. 28, 260–266 (2010). https://doi.org/10.1007/s00343-010-9229-5 DOI：

摘要

Abstract

Vibrio fluvialis incubated in trypticase soy broth (TSB) showed stronger adhesion to the skin mucus of Epinephelus awoara than V. fluvialis grown on trypticase soy agar (TSA)

and this bacterial adhesion was assessed in terms of saturation kinetics. Treating bacteria with antibody against O-antigens resulted in significantly reduced bacterial adhesion. In the early growth stage

the adhering bacteria numbers increased with incubation time

peaked at 24 h

and then dropped sharply. Prior heat treatment of the mucus at various temperatures resulted in different effects on bacterial adhesion. No remarkable effect on bacterial adhesion was detected after treating mucus with protease K and trypsin

whereas periodic acid treatment significantly enhanced bacterial adhesion. Extracts of V. fluvialis surface structures at high concentrations significantly inhibited bacterial adhesion

while relatively low concentrations enhanced adhesion. V. fluvialis had strong affinity for a high molecular weight mucus component. These results indicate that V. fluvialis adhered strongly to E. awoara mucus by specific adhesion and that this adhesion was influenced by many factors

including bacterial conditions

mucus

and the environment.

关键词

Keywords

references

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