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Tissue culture of three species of Laurencia complex
- Journal of Oceanology and Limnology Vol. 28, Issue 3, Pages: 514-520(2010)
- Affiliations：
1. College of Life Sciences Soochow University
2. Jiangsu Key Laboratory of Marine Biotechnology Huaihai Institute of Technology
- Author bio：
- Funds：
DOI：10.1007/s00343-010-9134-y
CLC：-
Online First：20 May 2010，
Published：2010
- Accepted：
Scan QR Code
Shen, S., Wu, X., Yan, B. et al. Tissue culture of three species of Laurencia complex., Chin. J. Ocean. Limnol. 28, 514–520 (2010). https://doi.org/10.1007/s00343-010-9134-y
摘要
Abstract
To establish a micropropagation system of three Laurencia complex species (Laurencia okamurai
Laurencia tristicha
and Chondrophycus undulatus) by tissue culture techniques
we studied the regeneration characteristics and optimal culture conditions of axenic algal fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under a reverse microscope to investigate the regeneration process
polarity and optimal illumination
and temperature and salinity levels. The results show that in most cultures of the three species
we obtained bud regeneration on solidified medium with 0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments in L. okamurai
but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species
and on fragments cut from the basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary and the cortical portions in L. okamurai and C. undulatus
while in L. tristicha
buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx
20°C and 35 (salinity); for C. undulatus
4 500 lx
20°C and 30; and for L. tristicha
4 500 lx
25°C and 30.
关键词
Keywords
references
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