PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)

Huaide Liu; Mei Liu; Baojie Wang; Keyong Jiang; Shan) Jiang; Shujuan Sun; Lei Wang

doi:10.1007/s00343-010-9101-7

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PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)

Updated：2023-05-16

- PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)
- Journal of Oceanology and Limnology Vol. 28, Issue 4, Pages: 808-814(2010)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate School of the Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9101-7
  CLC：
- Online First：29 June 2010，
  
  Published：2010
- Accepted：
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Liu, H., Liu, M., Wang, B. et al. PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)., Chin. J. Ocean. Limnol. 28, 808–814 (2010). https://doi.org/10.1007/s00343-010-9101-7
DOI：

Liu, H., Liu, M., Wang, B. et al. PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)., Chin. J. Ocean. Limnol. 28, 808–814 (2010). https://doi.org/10.1007/s00343-010-9101-7 DOI：

摘要

Abstract

In this study

the intestinal microbiota of kuruma shrimp (Marsupenaeus japonicus) was examined by molecular analysis of the 16S rDNA to identify the dominant intestinal bacteria and to investigate the effects of Bacillus spp. on intestinal microbial diversity. Samples of the intestines of kuruma shrimp fed normal feed and Bacillus spp. amended feed. PCR and denaturing gradient gel electrophoresis (DGGE) analyses were then performed on DNA extracted directly from the guts. Population fingerprints of the predominant organisms were generated by DGGE analysis of the universal V3 16S rDNA amplicons

and distinct bands in the gels were sequenced. The results suggested that the gut of kuruma shrimp was dominated by Vibrio sp. and uncultured gamma proteobacterium. Overall

the results of this study suggest that PCR-DGGE is a possible method of studying the intestinal microbial diversity of shrimp.

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references

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