Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch

Huicheng Yang; Mingyong Zeng; Shiyuan Dong; Zunying Liu; Ruixue Li

doi:10.1007/s00343-010-9054-x

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Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch

Updated：2023-05-16

- Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch
- Journal of Oceanology and Limnology Vol. 28, Issue 1, Pages: 122-130(2010)
- Affiliations：
  
  1. College of Food Science and Engineering Ocean University of China
  2. Zhejiang Marine Development Research Institute
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9054-x
  CLC：
- Online First：27 January 2010，
  
  Published：2010
- Accepted：
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Yang, H., Zeng, M., Dong, S. et al. Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch., Chin. J. Ocean. Limnol. 28, 122–130 (2010). https://doi.org/10.1007/s00343-010-9054-x
DOI：

Yang, H., Zeng, M., Dong, S. et al. Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch., Chin. J. Ocean. Limnol. 28, 122–130 (2010). https://doi.org/10.1007/s00343-010-9054-x DOI：

摘要

Abstract

In this study

we evaluated the anti-proliferative activity of phlorotannins derived from brown algae Laminaria japonica Aresch extracts on the human hepatocellular carcinoma cell (BEL-7402) and on murine leukemic cells (P388) by MTT assay. Cells were incubated with 100 μg/mL of the phlorotannin extract (PE) for 48 h. The inhibitory rate of PE on BEL-7402 and P388 cells was 30.20±1.16% and 43.44±1.86%

respectively

and the half-inhibitory concentration of PE (IC50) on P388 and BEL-7402 cells was 120 μg/mL and >200 μg/mL

respectively. Microscopic observation shows that the morphologic features of tumor cells treated with PE and 5-fluorouracil are markedly different from the normal control group. The inhibitory rate of fraction A2 isolated from PE by sephadex LH-20 for BEL-7402 and P388 cells at the sample concentration of 70.42 μg/mL was 61.96±7.02% and 40.47±8.70%

respectively. The apoptosis peak for fraction A2 was the most profound of all fractions used in the flow cytometry assay. The results indicate that the anti-proliferative of this algal extract is associated with the total phlorotannin content.

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references

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