Molecular phylogeny and species separation of five morphologically similar Holosticha-complex ciliates (Protozoa, Ciliophora) using ARDRA riboprinting and multigene sequence data

Feng Gao; Zhenzhen Yi; Jun Gong; A. S. Al-Rasheid Khaled; Weibo Song

doi:10.1007/s00343-010-9042-1

Your Location：

Home >

Browse articles >

Molecular phylogeny and species separation of five morphologically similar Holosticha-complex ciliates (Protozoa, Ciliophora) using ARDRA riboprinting and multigene sequence data

Updated：2023-05-16

- Molecular phylogeny and species separation of five morphologically similar Holosticha-complex ciliates (Protozoa, Ciliophora) using ARDRA riboprinting and multigene sequence data
- Journal of Oceanology and Limnology Vol. 28, Issue 3, Pages: 542-548(2010)
- Affiliations：
  
  1. Laboratory of Protozoology Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education College of Life Science South China Normal University
  2. Laboratory of Protozoology Institute of Evolution and Marine Biodiversity Ocean University of China
  3. Zoology Department King Saud University
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9042-1
  CLC：
- Online First：20 May 2010，
  
  Published：2010
- Accepted：
Scan QR Code
Gao, F., Yi, Z., Gong, J. et al. Molecular phylogeny and species separation of five morphologically similar Holosticha-complex ciliates (Protozoa, Ciliophora) using ARDRA riboprinting and multigene sequence data., Chin. J. Ocean. Limnol. 28, 542–548 (2010). https://doi.org/10.1007/s00343-010-9042-1
DOI：

Gao, F., Yi, Z., Gong, J. et al. Molecular phylogeny and species separation of five morphologically similar Holosticha-complex ciliates (Protozoa, Ciliophora) using ARDRA riboprinting and multigene sequence data., Chin. J. Ocean. Limnol. 28, 542–548 (2010). https://doi.org/10.1007/s00343-010-9042-1 DOI：

摘要

Abstract

To separate and redefine the ambiguous Holosticha-complex

a confusing group of hypotrichous ciliates

six strains belonging to five morphospecies of three genera

Holosticha heterofoissneri

Anteholosticha sp. pop1

Anteholosticha sp. pop2

A. manca

A. gracilis and Nothoholosticha fasciola

were analyzed using 12 restriction enzymes on the basis of amplified ribosomal DNA restriction analysis. Nine of the 12 enzymes could digest the DNA products

four (Hinf I

Hind III

Msp I

Taq I) yielded species-specific restriction patterns

and Hind III and Taq I produced different patterns for two Anteholosticha sp. populations. Distinctly different restriction digestion haplotypes and similarity indices can be used to separate the species. The secondary structures of the five species were predicted based on the ITS2 transcripts and there were several minor differences among species

while two Anteholosticha sp. populations were identical. In addition

phylogenies based on the SSrRNA gene sequences were reconstructed using multiple algorithms

which grouped them generally into four clades

and exhibited that the genus Anteholosticha should be a convergent assemblage. The fact that Holosticha species clustered with the oligotrichs and choreotrichs

though with very low support values

indicated that the topology may be very divergent and unreliable when the number of sequence data used in the analyses is too low.

关键词

Keywords

references

Berger H. 2003. Redefinition of Holosticha Wrzesniowski, 1877 (Ciliophora, Hypotricha). Eur. J. Protistol., 39: 373–379.

Berger H. 2006. Monograph of the Urostyloidea (Ciliophora, Hypotricha). Monogr. Biol., 85: 1–1 303.

Chen Z G, Song W B. 2002. Characterization and identification of the Diophrys species (Protozoa, Ciliophora, Hypotrichida) based on RAPD fingerprinting and ARDRA riboprinting. Eur. J. Protistol., 38: 383–391.

Clark C G, Martin D S, Diamond L S. 1995. Phylogenetic relationships among anuran trypanosomes as revealed by riboprinting. J. Eukaryot. Microbiol., 42: 92–96.

Coleman A W. 2003. ITS2 is a double-edged tool for eukaryote evolutionary comparisons. Trends. Genet., 19: 370–375.

Coleman A W. 2005. Paramecium aurelia revisited. J. Eukaryot. Microbiol., 52: 68–77.

Foissner W. 1996. Ontogenesis in ciliated protozoa with emphasis on stomatogenesis. In: Hausmann K, Bradbury P C ed. Ciliates, Cells as Organism. Gustav Fischer Verlag, Stuttgart, Jena, New York. p. 95–178.

Foissner W., Moon-van der Staay S Y, van der Staay G W M, Hackstein J H P, Krautgartner W D, Berger H. 2004. Reconciling classical and molecular phylogenies in the stichotrichines (Ciliophora, Spirotrichea), including new sequences from some rare species. Eur. J. Protistol., 40: 265–281.

Gong J, Gao S, Roberts D M, Al-Rasheid K A S, Song W B. 2008. Trichopodiella faurei n. sp (Ciliophora, Phyllopharyngea, Cyrtophoria): Morphological description and phylogenetic analyses based on SSU rRNA and group I intron sequences. J. Eukaryot. Microbiol., 55: 492–500.

Guo W B, Song W B, Al-Rasheid K A S, Shao C, Miao M, Al-Farraj S A, Al-Qurishy S A, Chen Z G., Yi Z Z, Gao S. 2008. Phylogenetic position of three Condylostoma species (Protozoa, Ciliophora, Heterotrichea) inferred from the small subunit rRNA gene sequence. Progr. Nat. Sci., 18: 1 089–1 093.

Hu X Z, Song W B. 2001. Morphology and morphogenesis of Holosticha heterofoissneri nov. spec. from the Yellow Sea, China (Ciliophora, Hypotrichida). Hydrobiologia, 448: 171–179.

Jerome C A, Lynn D H. 1996. Identifying and distinguishing sibling species in the Tetrahymena pyriformis complex (Ciliophora, Oligohymenophorea) using PCR/RFLP analysis of nuclear ribosomal DNA. J. Eukaryot. Microbiol., 43: 492–497.

Kahl A. 1932. Urtiere oder Protozoa. I: Wimpertiere oder Ciliata (Infusoria), 3. Spirotricha. Tierwelt. Dtl., 25: 399–650.

Li L F, Song W B, Shin M K, Warren A, Al-Rasheid K A S, Al-Khedhairy A A, Al-Arifi S. 2009a. Reconsideration of the phylogenetic positions of three stichotrichous genera Holosticha, Anteholosticha and Pseudokeronopsis (Spirotrichea: Ciliophora) inferred from complete SSU rRNA gene sequences. Progr. Nat. Sci., 19: 769–773.

Li L Q, Zhang Q Q, Hu X Z, Warren A, Al-Rasheid K A S, Al-Khedheiry A A, Jiang J M, Song W B. 2009b. A redescription of the marine hypotrichous ciliate, Nothoholosticha fasciola (Kahl, 1932) nov. gen., nov. comb. (Ciliophora: Urostylida) with brief notes on its cellular reorganization and SSrRNA gene sequence. Eur. J. Protistol., 45: 237–248.

Liu W W, Shao C, Gong J, Li J Q, Lin X F, Song W B. 2009. Morphology, morphogenesis and molecular phylogeny of a new marine urostylid ciliate (Ciliophora, Stichotrichia) from the South China Sea, and a brief overview of the convergent evolution of the midventral pattern within the Spirotrichea. Zool. J. Lin. Soci., (in press)

Miao M, Shao C, Jiang J M, Li L Q, Stoeck T, Song W B. 2009. Caryotricha minuta (Xu et al., 2008) nov. comb., a unique marine ciliate (Protista, Ciliophora, Spirotrichea) with phylogenetic estimation of the ambiguous genus Caryotricha inferred from small subunit rDNA sequence. Inter. J. Syst. Evol. Microb., 59: 430–438.

Nei M, Li W H. 1979. Mathematical mode for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA, 76: 5 269–5 273.

Rijk P D, Wachter R D. 1997. Rna Viz, a program for the visualisation of RNA secondary structure. Nucl. Acids. Res., 25: 4 679–4 684.

Schmidt S L, Bernhard D, Schlegela M, Foissner W. 2007. Phylogeny of the Stichotrichia (Ciliophora, Spirotrichea) reconstructed with nuclear small subunit rRNA gene sequences: discrepancies and accordance with morphological data. J. Eukaryot. Microbiol., 54: 201–209.

Strüder-Kypke M C, Lynn D H. 2003. Sequence analyses of the small subunit rRNA gene confirm the paraphyly of oligotrich ciliates sensu lato and support the monophyly of the subclasses Oligotrichia and Choreotrichia (Ciliophora, Spirotrichea). J. Zool., 260: 87–97.

Wetton J H, Carter R E, Parkin D T, Walters D. 1987. Demographic study of a wild house sparrow population by DNA fingerprinting. Nature, 327: 147–149.

Yi Z Z, Song W B, Shao C, Warren A, Al-Rasheid K A S, Roberts D, Miao M, Al-Quraishy S A, Chen Z G. 2008a. Phylogeny of some systematically uncertain urostyloids—Apokeronopsis, Metaurostylopsis, Thigmokeronopsis (Ciliophora, Stichotrichia) estimated with small subunit rRNA gene sequence information: Discrepancies and agreements with morphological data. Eur. J. Protistol., 44: 254–262.

Yi Z Z, Song W B, Warren A, Roberts D, Al-Rasheid K A S, Chen Z G., Al-Farraj S A, Hu X Z. 2008b. A molecular phylogenetic investigation of Pseudoamphisiella and Parabirojimia (Protozoa, Ciliophora, Spirotrichea), two genera with ambiguous systematic positions. Eur. J. Protistol., 44: 45–53.

Yi Z Z, Chen Z G, Warren A, Roberts D, Al-Rasheid K A S, Miao M, Gao S, Shao C, Song W B. 2008c. Molecular phylogeny of Pseudokeronopsis (Protozoa, Ciliophora, Urostylida), with reconsideration of three closely related species at inter- and intra-specific levels inferred from the small subunit ribosomal RNA (SSrRNA) gene and the ITS1-5.8S-ITS2 region sequences. J. Zool., 275: 268–275.

Yi Z Z, Clamp J, Al-Rasheid K A S, Al-Khedhairy A A, Chen Z G, Song W B. 2009. Evolutionary relationship and species separation of four morphologically similar stichotrichous ciliates (Protozoa, Ciliophora). Progr. Nat. Sci., 19: 581–586.

Zuker M. 2003. Fold Mweb server for nucleic acid folding and hybridization prediction. Nucl. Acids. Res., 31: 3 406–3 415.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Multi-locus phylogeny, morphological evolution and biogeography of Batrachospermaceae (Rhodophyta) based on multiple genetic sequences

Jaagichlorella menglunensis sp. nov. (Watanabeales, Chlorophyta), a new coccoid green alga isolated from China

Morphology and phylogeny of two species of Bathypathes from the tropical western Pacific, with description of a new species

Mitochondrial genomes of Tapes dorsatus and Cardita variegata: insights into Heteroconchia phylogeny

Lineaperpetua gen. nov.: a new diatom genus in the Thalassiosirales supported by morphology and molecular data

Related Author

Weinan GUO

Fangru NAN

Zhongshi HE

Xudong LIU

Yang LIU

Qi LIU

Jia FENG

Shulian XIE

Related Institution

School of Life Science, Shanxi Key Laboratory for Research and Development of Regional Plants, Shanxi University

Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore

School of Life Sciences, Jianghan University

Changjiang Basin Ecology and Environment Monitoring and Scientific Research Center, Changjiang Basin Ecology and Environment Administration, Ministry of Ecology and Environment

Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰