Comparison of ciliature microtubule organelles in three hypotrichous ciliate species

Yisong Li; Lei Shi; Fukang Gu

doi:10.1007/s00343-010-9038-x

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Comparison of ciliature microtubule organelles in three hypotrichous ciliate species

Updated：2023-05-16

- Comparison of ciliature microtubule organelles in three hypotrichous ciliate species
- Journal of Oceanology and Limnology Vol. 28, Issue 3, Pages: 443-450(2010)
- Affiliations：
  
  School of Life Science East China Normal University
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9038-x
  CLC：
- Online First：20 May 2010，
  
  Published：2010
- Accepted：
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Li, Y., Shi, L. & Gu, F. Comparison of ciliature microtubule organelles in three hypotrichous ciliate species., Chin. J. Ocean. Limnol. 28, 443–450 (2010). https://doi.org/10.1007/s00343-010-9038-x
DOI：

Li, Y., Shi, L. & Gu, F. Comparison of ciliature microtubule organelles in three hypotrichous ciliate species., Chin. J. Ocean. Limnol. 28, 443–450 (2010). https://doi.org/10.1007/s00343-010-9038-x DOI：

摘要

Abstract

We examined the structure and spatial organization of ciliature base-associated microtubules (BAM) in three hypotrichous ciliates (Stylonychia mytilus

Pseudourostyla cristata

Euplotes woodruffi) in fluorescence microscopy. The results revealed that BAM

including the anterior (ALM)

posterior longitudinal microtubule (PLM) and the transverse microtubule (TM) bands

are composed of tubulin. The respective microtubular bands have cytoplasmic polarization patterns that are significantly asymmetric. The BAM of the midventral files in P. cristata appear cord-shaped compared with the ALM bands of transverse cirri in both S. mytilus and E. woodruffi

which extend to the left anterior side of the cell before converging. The TM bands of the left marginal cirri (MC) in S. mytilus extend to the right side of the cell

while those of the right MC bands extend to the left. Our observations suggest that BAM traits are common in hypotrichous ciliates even though different species possess different microtubule arrangements related to the conserved cirral morphogenetic patterns in the respective species. The differing development of BAM in the three ciliate suggests that the microtubules may be conserved in different hypotrichs. We have also demonstrated that the BAM

which appear polar and asymmetric

are localized in specific cytoskeletal positions and extend in different orientations within the cortex to connect with other ciliature-associated structures and

thus

strengthen the cortex. These BAM features indicate that they are directly associated with cell motion.

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references

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