Cloning and stage-specific expression of CK-M1 gene during metamorphosis of Japanese flounder, Paralichthys olivaceus

Yanjie Chen; Quanqi Zhang; Jie Qi; Zhigang Wang; Xubo Wang; Yeying Sun; Qiwang Zhong; Shuo Li; Chunmei Li

doi:10.1007/s00343-010-9023-4

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Cloning and stage-specific expression of CK-M1 gene during metamorphosis of Japanese flounder, Paralichthys olivaceus

Updated：2023-05-16

- Cloning and stage-specific expression of CK-M1 gene during metamorphosis of Japanese flounder, Paralichthys olivaceus
- Journal of Oceanology and Limnology Vol. 28, Issue 3, Pages: 558-564(2010)
- Affiliations：
  
  Laboratory of Marine Genetics and Breeding Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9023-4
  CLC：
- Online First：20 May 2010，
  
  Published：2010
- Accepted：
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Chen, Y., Zhang, Q., Qi, J. et al. Cloning and stage-specific expression of CK-M1 gene during metamorphosis of Japanese flounder, Paralichthys olivaceus, ., Chin. J. Ocean. Limnol. 28, 558–564 (2010). https://doi.org/10.1007/s00343-010-9023-4
DOI：

Chen, Y., Zhang, Q., Qi, J. et al. Cloning and stage-specific expression of CK-M1 gene during metamorphosis of Japanese flounder, Paralichthys olivaceus, ., Chin. J. Ocean. Limnol. 28, 558–564 (2010). https://doi.org/10.1007/s00343-010-9023-4 DOI：

摘要

Abstract

The symmetrical body of flatfish larvae changes dramatically into an asymmetrical form after metamorphosis. The molecular mechanisms responsible for this change are poorly understood. As an initial step to clarify these mechanisms

we used representational difference analysis of cDNA for the identification of genes active during metamorphosis in the Japanese flounder

Paralichthys olicaceus. One of the up-regulated genes was identified as creatine kinase muscle type 1 (CK-M1). Sequence analysis of CK-M1 revealed that it spanned 1 708 bp and encoded a protein of 382 amino acids. The overall amino acid sequence of the CK-M1 was highly conserved with those of other organisms. CK-M1 was expressed in adult fish tissues

including skeletal muscle

intestine and gill. Whole mount in-situ hybridization showed that the enhanced expression of CK-M1 expanded from the head to the whole body of larvae as metamorphosis progressed. Quantitative analysis revealed stage-specific high expression of CK-M1 during metamorphosis. The expression level of CK-M1 increased initially and peaked at metamorphosis

decreased afterward

and finally returned to the pre-metamorphosis level. This stage-specific expression pattern suggested strongly that CK-M1 was related to metamorphosis in the Japanese flounder. Its specific role in metamorphosis requires further study.

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Keywords

references

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