Steroid sex hormone dynamics during estradiol-17β induced gonadal differentiation in Paralichthys olivaceus (Teleostei)

Peng Sun; Feng You; Mengxia Liu; Zhihao Wu; Aiyun Wen; Jun Li; Yongli Xu; Peijun Zhang

doi:10.1007/s00343-010-9011-8

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Steroid sex hormone dynamics during estradiol-17β induced gonadal differentiation in Paralichthys olivaceus (Teleostei)

Updated：2023-05-16

- Steroid sex hormone dynamics during estradiol-17β induced gonadal differentiation in Paralichthys olivaceus (Teleostei)
- Journal of Oceanology and Limnology Vol. 28, Issue 2, Pages: 254-259(2010)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate School of Chinese Academy of Sciences
  3. Shandong Provincial Mari-culture Institute
- Author bio：
- Funds：
- DOI：10.1007/s00343-010-9011-8
  CLC：
- Online First：20 March 2010，
  
  Published：2010
- Accepted：
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Sun, P., You, F., Liu, M. et al. Steroid sex hormone dynamics during estradiol-17β induced gonadal differentiation in Paralichthys olivaceus (Teleostei)., Chin. J. Ocean. Limnol. 28, 254–259 (2010). https://doi.org/10.1007/s00343-010-9011-8
DOI：

Sun, P., You, F., Liu, M. et al. Steroid sex hormone dynamics during estradiol-17β induced gonadal differentiation in Paralichthys olivaceus (Teleostei)., Chin. J. Ocean. Limnol. 28, 254–259 (2010). https://doi.org/10.1007/s00343-010-9011-8 DOI：

摘要

Abstract

Steroid sex hormones

such as estradiol-17β (E2) and testosterone (T)

are important regulators of sex change in fish. In this study

we examined the effects of E2 treatment on the dynamics of E2 and T during gonadal differentiation in the olive flounder Paralichthys olivaceus using histology and radioimmunoassay (RIA). Flounder larvae were divided into five groups (G0-G4)

and fed with 0 (control)

0.2

20 and 100 mg E2/kg feed from 35 to 110 day post hatching (dph). Fish growth in the G1 and G2 groups was not significantly different from that of the control group (P>0.05)

while fish in the G3 and G4 groups were less active and showed growth depression and high mortality. The gonads of fish in the G3 and G4 groups were smaller and surrounded by hyperplastic connective tissue. The frequency of females in the G0-G4 groups was 54.5%

75.0%

100%

100% and 93.3%

respectively. The RIA analyses of E2 and T showed that T levels decreased during gonadal differentiation

and increased slightly at the onset of ovarian differentiation

while E2 levels increased gradually and peaked at the onset of ovarian differentiation in the control group. In the E2-treated groups

T levels decreased before the onset of ovarian differentiation. E2 levels were high on the 48 dph

but declined to a lower level on the 54 dph

and then increased gradually during gonadal differentiation. And a sharp increase of E2 levels were observed in all E2-treated groups at the onset of ovarian differentiation. The data suggest that T and E2 play important roles during gonadal differentiation

and an E2 dose of 2 mg/kg feed could induce sex reversal in P. olivaceus.

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references

Baron D, Fostier A, Breton B, Guiguen Y. 2005. Androgen and estrogen treatments alter steady state messengers RNA (mRNA) levels of testicular steroidogenic enzymes in the rainbow trout, Oncorhynchus mykiss. Molecular Reproduction and Development, 71: 471–479.

Chang C F, Lan S C, Chou H Y. 1995. Gonadal histology and plasma sex steroids during sex differentiation in grey mullet, Mugil cephalus. Journal of Experimental Zoology, 272: 395–406.

Devlin R H, Nagahama Y. 2002. Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture, 208: 191–364.

Du J L, Lin B Y, Lee Y H, Yueh W S, He C L, Lee M F, Sun L T, Chang C F. 2003. Estradiol, aromatase and steroid receptors involved in the sex change of protandrous black porgy, Acanthopagrus schlegeli. Fish Physiology and Biochemistry, 28: 131–133.

Fitzpatrick M S, Pereira C B, Schreck C B. 1993. In vitro steroid secretion during early development of mono-sex rainbow trout: sex differences, onset of pituitary control, and effects of dietary steroid treatment. General and Comparative Endocrinology, 91: 199–215.

Fostier A, Jalabert B. 1986. Steroidogenesis in rainbow trout (Salmo gairdneri) at various preovulatory stages: changes in plasma hormone levels and in vivo and in vitro response of the ovary to salmon gonadotropin. Fish Physiology and Biochemistry, 2: 87–90.

Govoroun M, McMeel O M, Mecherouki H, Smith T J, Guiguen Y. 2001. 17beta-estradiol treatment decreases steroidogenic enzyme messenger ribonucleic acid levels in the rainbow trout testis. Endocrinology, 142: 1 841–1 848.

Hines G A, Boots L R, Wibbels T, Watts S A. 1999. Steroid levels and steroid metabolism in relation to early gonadal development in the tilapia Oreochromis niloticus (Teleostei: Cyprinoidei). General and Comparative Endocrinology, 114(2): 235–248.

Hirai N, Nanba A, Koshio M, Kondo T, Morita M, Tatarazako N. 2006. Feminization of Japanese medaka (Oryzias latipes) exposed to 17β-estradiol: formation of testis-ova and sex-transformation during early-ontogeny. Aquatic Toxicology, 77: 78–86.

Kim D S, Nam Y K, Jo J Y. 1997. Effect of oestradiol-17β immersion treatment on sex reversal of mud loach, Misgurnus mizolepis (Günther). Aquaculture Research, 28: 941–946.

Kojima Y, Bhandari R K, Kobayashi Y, Nakamura M. 2008. Sex change of adult initial-phase male wrasse, Halichoeres trimaculatus by estradiol-17β treatment. General and Comparative Endocrinology, 156: 628–632.

Labadie P, Budzinski H. 2006. Alteration of steroid hormone profile in juvenile turbot (Psetta maxima) as a consequence of short-term exposure to 17α-ethynylestradiol. Chemosphere, 64: 1 274–1 286.

Nakamura M, Nagahama Y. 1985. Steroid producing cells during ovarian differentiation of the tilapia, Sarotherodon niloticus. Development Growth and Differentiation, 27: 701–708.

Nakamura M, Nagahama Y. 1993. Ultrastructural study on the differentiation and development of steroid-producing cells during ovarian differentiation in the amago salmon, Oncorhynchus rhodurus. Aquaculture, 112: 237–251.

Nakamura M, Bhandari R K, Higa M. 2003. The role estrogens play in sex differentiation and sex changes of fish. Fish Physiology and Biochemistry, 28: 113–117.

Park I S, Kim J H, Cho S H, Kim D S. 2004. Sex differentiation and hormonal sex reversal in the bagrid catfish Pseudobagrus fulvidraco (Richardson). Aquaculture, 232: 183–193.

Piferrer F, Donaldson E M. 1992. The comparative effectiveness of the natural and a synthetic estrogen for the direct feminization of chinook salmon (Oncorhynchus tashawytscha). Aquaculture, 106: 183–193.

Piferrer F. 2001. Endocrine sex control strategies for the feminization of teleost fish. Aquaculture, 197: 229–281.

Rothbard S, Yaron Z. 1987. Changes in steroids concentrations during sexual ontogenesis in tilapia. Aquaculture, 61: 59–74.

Rougeot C, Krim A, Mandiki S N M, Kestemont P, Mélard C. 2007. Sex steroid dynamics during embryogenesis and sexual differentiation in Eurasian perch, Perca fluviatilis. Theriogenology, 67: 1 046–1 052.

Rougeot C, Jacobs B, Kestemont P, Mélard C. 2002. Sex control and sex determinism study in Eurasian perch, Perca fluviatilis, by use of hormonally sex-reversed male breeders. Aquaculture, 211: 81–89.

Tabata K. 1989. Artificial feminization of hirame Paralichthys olivaceus by administration of beta-estradiol, and estimation of stage of sexual differentiation. Bulletin of Hyogo Prefectural Fisheries Experimental Station, 26: 19–36.

Tanaka H. 1988. Effect of estradiol-17 beta on gonadal sex differentiation in flounder, Paralichthys olivaceus. Bulletin of National Research Institute of Aquaculture, 13: 17–23.

Yamamoto T. 1969. Sex differentiation. In: Hoar W S, Randall D J ed. Fish physiology; reproduction and growth bioluminescence pigments and poisons, Vol. III. Academic Press, New York. p. 117–175.

Yamamoto E. 1995. Studies on sex-manipulation and production of cloned populations in hirame, Paralichthys olivaceus (Temminck et Sehlege1). Bulletin of the Tottori Prefectual Fisheries Experimental Station, 34: 1–145.

Yamamoto E. 1999. Studies on sex-manipulation and production of cloned populations in hirame, Paralichthys olivaceus (Temminck et Sehlege1). Aquaculture, 173: 235–246.

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