Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum

Yifan Han; Zhaolan Mo; Yunxiang Mao; Peng Xiao; Jie Li; Bin Hao; Dongsheng Guo

doi:10.1007/s00343-009-9230-z

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Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum

Updated：2023-05-16

- Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum
- Journal of Oceanology and Limnology Vol. 27, Issue 3, Pages: 519-526(2009)
- Affiliations：
  
  1. Ocean University of China
  2. Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9230-z
  CLC：
- Online First：07 November 2009，
  
  Published：2009
- Accepted：
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Han, Y., Mo, Z., Mao, Y. et al. Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum, ., Chin. J. Ocean. Limnol. 27, 519–526 (2009). https://doi.org/10.1007/s00343-009-9230-z
DOI：

Han, Y., Mo, Z., Mao, Y. et al. Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum, ., Chin. J. Ocean. Limnol. 27, 519–526 (2009). https://doi.org/10.1007/s00343-009-9230-z DOI：

摘要

Abstract

Vibrio anguillarum is a common bacterial pathogen in fish. However

little is known about its pathogenic mechanism

in part

because the entire genome has not been completely sequenced. We constructed a fosmid library for V. anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage. We characterized the library by end-sequencing 50 randomly selected clones. This generated 93 sequences with a total length of 57 485 bp covering 1.4% of the whole genome. Of these sequences

58 (62.4%) were homologous to known genes

30 (32.3%) were genes with hypothetical functions

and the remaining 5 (5.3%) were unknown genes. We demonstrated the utility of this library by PCR screening of 10 genes. This resulted in an average of 6.2 fosmid clones per screening. This fosmid library offers a new tool for gene screening and cloning of V. anguillarum

and for comparative genomic studies among Vibrio species.

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references

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