Simultaneous enumeration of diatom, protozoa and meiobenthos from marine sediments using Ludox-QPS method

Yongfen Du; Kuidong Xu; Yanli Lei

doi:10.1007/s00343-009-9224-x

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Simultaneous enumeration of diatom, protozoa and meiobenthos from marine sediments using Ludox-QPS method

Updated：2023-05-16

- Simultaneous enumeration of diatom, protozoa and meiobenthos from marine sediments using Ludox-QPS method
- Journal of Oceanology and Limnology Vol. 27, Issue 4, Pages: 775-783(2009)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate School Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9224-x
  CLC：
- Online First：04 December 2009，
  
  Published：2009
- Accepted：
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Du, Y., Xu, K. & Lei, Y. Simultaneous enumeration of diatom, protozoa and meiobenthos from marine sediments using Ludox-QPS method., Chin. J. Ocean. Limnol. 27, 775–783 (2009). https://doi.org/10.1007/s00343-009-9224-x
DOI：

Du, Y., Xu, K. & Lei, Y. Simultaneous enumeration of diatom, protozoa and meiobenthos from marine sediments using Ludox-QPS method., Chin. J. Ocean. Limnol. 27, 775–783 (2009). https://doi.org/10.1007/s00343-009-9224-x DOI：

摘要

Abstract

The Ludox-QPS method is a newly developed technique

which combines the Ludox HS 40 density centrifugation and quantitative protargol stain

to enumerate marine ciliates with good taxonomic resolution. We tested the method for simultaneous enumeration of diatoms

protozoa and meiobenthos and compared its extraction efficiency for meiobenthos with that of the routine Ludox-TM centrifugation and a modified protocol using Ludox HS 40. We conducted the evaluation with a sample size of 8.3 ml each from sandy

muddy-sand and muddy sediments collected from the intertidal area of the Yellow Sea in summer 2006 and spring 2007. The Ludox-QPS method not only produced high extraction efficiencies of 97±1.3% for diatoms and 97.6±0.8% for ciliates

indicating a reliable enumeration for eukaryotic microbenthos

but also produced excellent extraction efficiencies of on average 97.3% for total meiobenthos

97.9% for nematodes and 97.8% for copepods from sands

muddy sands and mud. By contrast

the routine Ludox-TM centrifugation obtained only about 74% of total meiobenthos abundance with one extraction cycle

and the modified Ludox HS 40 centrifugation yielded on average 93% of total meiobenthos: 89.4±2.0% from sands

93±4.1% from muddy sands and 97.1±3.0% from mud. Apart from the sediment type

sample volume was another important factor affecting the extraction efficiency for meiobenthos. The extraction rate was increased to about 96.4% when using the same modified Ludox centrifugation for a 4 ml sediment sample. Besides the excellent extraction efficiency

the Ludox-QPS method obtained higher abundances of meiobenthos

in particular nematodes

than the routine Ludox centrifugation

which frequently resulted in an uncertain loss of small meiobenthos during the sieving process. Statistical analyses demonstrated that there were no significant differences between the meiobenthos communities revealed by the Ludox-QPS method and the modified Ludox HS 40 centrifugation

showing the high efficiency of the Ludox-QPS method for simultaneous enumeration of diatom

protozoa and meiobenthos. Moreover

the comparatively high taxonomic resolution of the method

especially for diatoms and ciliates

makes it feasible to investigate microbial ecology at community level.

关键词

Keywords

references

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