Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae

Wenbin Zhan; Hongming Liu; Jing Xing; Xiuzhen Sheng; Xiaoqian Tang

doi:10.1007/s00343-009-9195-y

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Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae

Updated：2023-05-16

- Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae
- Journal of Oceanology and Limnology Vol. 27, Issue 3, Pages: 536-542(2009)
- Affiliations：
  
  Laboratory of Pathology and Immunology of Aquatic Animals LMMEC Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9195-y
  CLC：
- Online First：07 November 2009，
  
  Published：2009
- Accepted：
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Zhan, W., Liu, H., Xing, J. et al. Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae, ., Chin. J. Ocean. Limnol. 27, 536–542 (2009). https://doi.org/10.1007/s00343-009-9195-y
DOI：

Zhan, W., Liu, H., Xing, J. et al. Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae, ., Chin. J. Ocean. Limnol. 27, 536–542 (2009). https://doi.org/10.1007/s00343-009-9195-y DOI：

摘要

Abstract

A pathogenic bacterium (S636)

identified as Streptococcus iniae

was isolated from turbot (Scophthalmus maximus) in 2005. We immunized turbot with formalin-killed S. iniae four times (on days 1

and 28) by intraperitoneal inoculation. After each vaccination

we obtained serum samples and isolated the lymphocytes from the peripheral blood

spleen

pronephros

and mesonephros. We measured surface Ig-positive (sIg+) lymphocytes and serum antibody levels from these organs using flow cytometry and enzyme-linked immunosorbent assay (ELISA)

respectively

using monoclonal antibodies against turbot immunoglobulin. We confirmed that the antibody reacted with both the surface and plasma Ig by confocal laser scanning microscopy and electron microscopy. The percentage of sIg+ in the lymphocytes increased following each successive vaccination. The mean percentage increased from 31.96% (control) to 37.49%

38.36%

42.9%

and 51.63% in the peripheral blood; from 27.09% to 36.63%

36.81%

39.28%

and 46.0% in the spleen; from 22.2% to 28.99%

29.21%

32.83%

and 41.58% in pronephros; and from 18.12% to 22.17%

22.45%

25.69%

and 31.68% in the mesonephros. The ELISA results were consistent with these results. Both the total and specific antibody levels increased with each vaccination. The mean OD value of the specific antibody assay increased from 0.094

to 0.269

0.283

0.333

and 0.421; for total antibody the mean OD value increased from 0.133

to 0.292

0.323

0.413

and 0.527.

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Keywords

references

Breuil G, Vassiloglou B, Pepin J F, Romestand B. 1997. Ontogeny of IgM-bearing cells and changes in the immunoglobulin M-like protein level (IgM) during larval stage in sea bass (Dicentrarchus labrax). Fish Shellfish Immunol., 7: 29–43.

Holt J G, Krieg N R, Sneath P H A, Staley J T, Williams S T. 1999. Bergey’s Manual of Determinative Bacteriology (ninth edition). Landon: Williams & Wilkins. p. 532–559.

Hrubec T C, Ward D, Smith S A, Robertson J L. 2004. Age related changes in humoral immune response of hybrid bass (Morone chrysops×Morone saxatilis). Vet. Immunol. Immunopathol., 101: 103–108.

Johnson K A, Flynn J K, Amend D F. 1982. Duration of immunity in salmonids vaccinated by direct immersion in Vibrio anguillarum and Yersinia ruckeri bacterins. J Fish Dis., 5: 197–205.

Kitao T. 1993. Streptococcal infections. In: Inglis V, Roberts R J, Bromage N R (eds) Bacterial Diseases in Fish. Blackwell, Oxford.

Magnadòttir B, Kristjansdottir H, Gudmundsdottir S. 1996. Characterisation of monocolonal antibodies to separate epitopes on salmon IgM heavy chain. Fish Shellfish Immunol., 6: 185–198.

McNulty S T, Klesius P H, Shoemaker C A, Evans J J. 2003. Streptococcus iniae infection and tissue distribution in hybrid bass (Morone chrysops × Morone saxatilis) following inoculation of the gills. Aquaculture, 220: 165–173.

Miyadai T, Ootani M, Tahara D, Aoki M, Saitoh K. 2004. Monoclonal antibodies recognizing serum immunoglobulins and surface immunoglobulin-positive cells of puffer fish, torafugu (Takifugu rubripes). Fish Shellfish Immunol., 17: 211–222.

Nguyen H T, Kinya K, Kazuma Y. 2002. Ecological investigation of Streptococcus iniae in cultured Japanese flounder (Paralichthys olivaceus) using selective isolation procedures. Aquaculture, 205: 7–17.

Panigrahi A, Kiron V, Puangkaew J, Kobayashi T, Satoh S, Sugita H. 2005. The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Oncorhynchus mykiss. Aquaculture, 243: 241–254.

Quentel C, Ogier de Baulny M. 1995. Vaccination of juvenile turbot, Scophthalmus maximus L. against vibriosis. Aquaculture, 132: 125–131.

Salvador A, Carmen B, Eduardo M M, Miguel A M. 2004. Immune response of gilt-head seabream (Sparus aurata) to antigens from Photobacterium damselae subsp. Piscicida. Fish Shellfish Immunol., 16: 65–70.

Satoh K, Fukuda Y, Nakaro S. 1995. Changes in agglutinin titer against Pasteurella pisicicida in cultured yellowtail during the epizootics of pseudotuberculosis in 1993 and 1994. Fish Pathol., 30: 291–292.

Van Diepen J C E K, Wagenaar G T M, Rombout J H W M. 1991. Immunocytochemical detection of membrane antigens of carp leucocytes using light and electron microscopy. Fish Shellfish Immunol., 1: 47–57.

Van der Heijden M H T, Rooijakkers J B M A, Booms G H R, Rombout J H W M, Boon J H. 1995. Production, characterisation and applicability of monoclonal antibodies to European eel (Anguilla anguilla L., 1758) immunoglobulin. Vet. Immunol. Immunopathol., 45: 151–164.

Van Diepen J C E K, Thiele J J T, Van Rens B T T M, Rombout J H W M. 1994. Immunocytochemical and flow cytometric analysis of B cells and plasma cells in carp (Cyprins carpio L.); an ontogenetic study. Fish Shellfish Immunol., 4: 19–28.

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