The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity

Yongchao Guo; Zhenxing Li; Hong Lin

doi:10.1007/s00343-009-9190-3

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The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity

Updated：2023-05-16

- The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity
- Journal of Oceanology and Limnology Vol. 27, Issue 4, Pages: 703-707(2009)
- Affiliations：
  
  Food Safety Laboratory Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9190-3
  CLC：
- Online First：04 December 2009，
  
  Published：2009
- Accepted：
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Guo, Y., Li, Z. & Lin, H. The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity., Chin. J. Ocean. Limnol. 27, 703–707 (2009). https://doi.org/10.1007/s00343-009-9190-3
DOI：

Guo, Y., Li, Z. & Lin, H. The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity., Chin. J. Ocean. Limnol. 27, 703–707 (2009). https://doi.org/10.1007/s00343-009-9190-3 DOI：

摘要

Abstract

Pen a 1 is a major shrimp allergen that induces an allergy. The aim of this study is to assess the effect of simulated gastrointestinal fluids on the allergenicity of Pen a 1. Purified Pen a 1 from shrimp (Penaeus vannamei) was subjected to digestion in SGF (simulated gastric fluid) and SIF (simulated intestinal fluid) for a set time. The allergenicity of digestive Pen a 1 was analyzed by immunoblotting and Ci-ELISA

using pool sera from patients with shrimp specific IgE. The results showed that Pen a 1 exhibited a decrease in allergenicity with increasing digestion time in the SGF and SIF. However

Pen a l exhibited strong resistance to digestive fluids

and all yielded fragments (33 kD

23 kD

and 14 kD) showed allergic activity. Therefore

anti-digestion may be an important factor for Pen a 1 to induce an allergy.

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references

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