Antiangiogenic activity of low-temperature lysozyme from a marine bacterium in vivo and in vitro

Zhenhua Wang; Jincheng Liu; Ai Su; Mi Sun; Chunbo Wang

doi:10.1007/s00343-009-9181-4

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Antiangiogenic activity of low-temperature lysozyme from a marine bacterium in vivo and in vitro

Updated：2023-05-16

- Antiangiogenic activity of low-temperature lysozyme from a marine bacterium in vivo and in vitro
- Journal of Oceanology and Limnology Vol. 27, Issue 4, Pages: 835-844(2009)
- Affiliations：
  
  1. Medical College Qingdao University
  2. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9181-4
  CLC：
- Online First：04 December 2009，
  
  Published：2009
- Accepted：
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Wang, Z., Liu, J., Su, A. et al. Antiangiogenic activity of low-temperature lysozyme from a marine bacterium in vivo and in vitro, ., Chin. J. Ocean. Limnol. 27, 835–844 (2009). https://doi.org/10.1007/s00343-009-9181-4
DOI：

Wang, Z., Liu, J., Su, A. et al. Antiangiogenic activity of low-temperature lysozyme from a marine bacterium in vivo and in vitro, ., Chin. J. Ocean. Limnol. 27, 835–844 (2009). https://doi.org/10.1007/s00343-009-9181-4 DOI：

摘要

Abstract

We extracted marine low-temperature lysozyme (MLTL)

a novel lysozyme

from a marine microorganism through fermentation. Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this study

we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model

we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 µg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation

migration and tube formation of HUVECs in a dose-dependent manner. Interestingly

assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs. Moreover

further study demonstrated that the disruption of intracellular Ca2+ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together

the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca2+ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.

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references

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