A polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via the Apaf-1/caspase-9 and Smac/XIAP signaling pathway

Xiaojin Liu; Wencheng Wang; Hongjiang Wang; Lanlan Zhang; Leqian Liu; Yuejun Wang; Chunbo Wang

doi:10.1007/s00343-009-9179-y

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A polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via the Apaf-1/caspase-9 and Smac/XIAP signaling pathway

Updated：2023-05-16

- A polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via the Apaf-1/caspase-9 and Smac/XIAP signaling pathway
- Journal of Oceanology and Limnology Vol. 27, Issue 3, Pages: 587-593(2009)
- Affiliations：
  
  1. Department of Pharmacology Medical College of Qingdao University
  2. Medical College Taishan University
  3. Department of Biological Sciences East Tennessee State University
  4. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9179-y
  CLC：
- Online First：07 November 2009，
  
  Published：2009
- Accepted：
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Liu, X., Wang, W., Wang, H. et al. A polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via the Apaf-1/caspase-9 and Smac/XIAP signaling pathway., Chin. J. Ocean. Limnol. 27, 587–593 (2009). https://doi.org/10.1007/s00343-009-9179-y
DOI：

Liu, X., Wang, W., Wang, H. et al. A polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via the Apaf-1/caspase-9 and Smac/XIAP signaling pathway., Chin. J. Ocean. Limnol. 27, 587–593 (2009). https://doi.org/10.1007/s00343-009-9179-y DOI：

摘要

Abstract

A novel marine active polypeptide (PCF)

isolated from the gonochoric Chinese scallop

Chlamys farreri

has potential antioxidant and anti-apoptotic activity against ultraviolet irradiation. We investigated whether UVB-induced HaCaT cell apoptosis occurs via the mitochondrial pathways Apaf-1/caspase-9 and Smac/XIAP/caspase-3. We then investigated the molecular mechanisms controlling the anti-apoptotic effect of PCF. Pre-treatment with PCF and caspase-9 inhibitor significantly inhibited UVB-induced apoptosis in HaCaT cells based on a DNA fragmentation assay and Hoechst 33258 staining. The expression of Apaf-1 and the cleavage of procaspase-9 were dose-dependently reduced by 1.42–5.96 mmol/L PCF pretreatment in UVB-irradiated HaCaT cells. This was followed by inhibition of cleavage of procaspase-3

whose activation induced cell apoptosis. Meanwhile

PCF significantly and dose-dependently enhanced the activation of ATPase. Furthermore

we demonstrated that PCF strongly inhibited the release of Smac from the mitochondria to cytosol by reducing the degradation of XIAP dose-dependently. We conclude that the protective effect of PCF against UVB irradiation in HaCaT cells may be attributed to the inhibition of the Apaf-1/caspase-9 and Smac/XIAP/caspase-3 apoptotic signaling pathways.

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