Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via iNOS/NO and HSP90

Zhengyang Zhang; Xiaojin Liu; Tuo Liu; Lin Yan; Yuejun Wang; Chunbo Wang

doi:10.1007/s00343-009-9171-6

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Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via iNOS/NO and HSP90

Updated：2023-05-16

- Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via iNOS/NO and HSP90
- Journal of Oceanology and Limnology Vol. 27, Issue 3, Pages: 594-599(2009)
- Affiliations：
  
  1. Department of Pharmacology Medical College of Qingdao University
  2. Department of Public Health Medical College of Qingdao University
  3. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9171-6
  CLC：
- Online First：07 November 2009，
  
  Published：2009
- Accepted：
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Zhang, Z., Liu, X., Liu, T. et al. Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via iNOS/NO and HSP90., Chin. J. Ocean. Limnol. 27, 594–599 (2009). https://doi.org/10.1007/s00343-009-9171-6
DOI：

Zhang, Z., Liu, X., Liu, T. et al. Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via iNOS/NO and HSP90., Chin. J. Ocean. Limnol. 27, 594–599 (2009). https://doi.org/10.1007/s00343-009-9171-6 DOI：

摘要

Abstract

Polypeptide from Chlamys farreri (PCF) is a novel marine bioactive product that was isolated from the gonochoric Chinese scallop Chlamys farreri

and was found to be an effective antioxidant in our recent studies. In this study

we investigated the effect of PCF on ultraviolet B (UVB)-induced apoptosis of HaCaT cells and the intracellular signaling pathways involved. Pretreatment with the inducible nitric oxide synthase (iNOS) inhibitor S-methylisothiourea sulfate inhibited UVB-induced apoptosis

indicating that iNOS and NO play important roles in apoptosis. On the other hand

the inhibition of UVB-induced apoptosis in the immortalized keratinocyte (HaCaT) cells by PCF was estimated using a DNA ladder. PCF treatment inhibited UVB-induced iNOS activation

as determined by RT-PCR

NO production

as determined by ESR

and up-regulated heat shock protein (HSP) 90 activation

as determined by Western blotting. Our results indicate that iNOS and NO are involved in UVB-induced apoptosis of HaCaT cells and the protective effect of PCF against UVB irradiation is exerted by suppressing the expression of iNOS

followed by inhibition of NO release and enhanced activation of HSP90.

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references

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