An improved PCR method for direct identification of Porphyra (Bangiales, Rhodophyta) using conchocelis based on a RUBISCO intergenic spacer

Chao Wang; Dong Dong; Guangce Wang; Baoyu Zhang; Guang Peng; Pu Xu; Xiaorong Tang

doi:10.1007/s00343-009-9169-0

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An improved PCR method for direct identification of Porphyra (Bangiales, Rhodophyta) using conchocelis based on a RUBISCO intergenic spacer

Updated：2023-05-16

- An improved PCR method for direct identification of Porphyra (Bangiales, Rhodophyta) using conchocelis based on a RUBISCO intergenic spacer
- Journal of Oceanology and Limnology Vol. 27, Issue 3, Pages: 513-518(2009)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
  3. Tianjin University of Science and Technology
  4. Changshu Institute of Technology
  5. Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9169-0
  CLC：
- Online First：07 November 2009，
  
  Published：2009
- Accepted：
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Wang, C., Dong, D., Wang, G. et al. An improved PCR method for direct identification of Porphyra (Bangiales, Rhodophyta) using conchocelis based on a RUBISCO intergenic spacer., Chin. J. Ocean. Limnol. 27, 513–518 (2009). https://doi.org/10.1007/s00343-009-9169-0
DOI：

Wang, C., Dong, D., Wang, G. et al. An improved PCR method for direct identification of Porphyra (Bangiales, Rhodophyta) using conchocelis based on a RUBISCO intergenic spacer., Chin. J. Ocean. Limnol. 27, 513–518 (2009). https://doi.org/10.1007/s00343-009-9169-0 DOI：

摘要

Abstract

An improved method of PCR in which the small segment of conchocelis is amplified directly without DNA extraction was used to amplify a RUBISCO intergenic spacer DNA fragment from nine species of red algal genus Porphyra (Bangiales

Rhodophyta)

including Porphyra yezoensis (Jiangsu

China)

P. haitanensis (Fujian

China)

P. oligospermatangia (Qingdao

China)

P. katadai (Qingdao

China)

P. tenera (Qingdao

China)

P. suborboculata (Fujian

China)

P. pseudolinearis (Kogendo

Korea)

P. linearis (Devon

England)

and P. fallax (Seattle

USA). Standard PCR and the method developed here were both conducted using primers specific for the RUBISCO spacer region

after which the two PCR products were sequenced. The sequencing data of the amplicons obtained using both methods were identical

suggesting that the improved PCR method was functional. These findings indicate that the method developed here may be useful for the rapid identification of species of Porphyra in a germplasm bank. In addition

a phylogenetic tree was constructed using the RUBISCO spacer and partial rbcS sequence

and the results were in concordant with possible alternative phylogenies based on traditional morphological taxonomic characteristics

indicating that the RUBISCO spacer is a useful region for phylogenetic studies.

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Keywords

references

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