Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation

Yuxi Wei; Changyun Wang; Jing Li; Qi Guo; Hongtao Qi

doi:10.1007/s00343-009-9166-3

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Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation

Updated：2023-05-16

- Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation
- Journal of Oceanology and Limnology Vol. 27, Issue 3, Pages: 558-563(2009)
- Affiliations：
  
  1. Biological Department Qingdao University
  2. Key Laboratory of Marine Drugs Ministry of Education School of Medicine and Pharmacy Ocean University of China
  3. Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9166-3
  CLC：
- Online First：07 November 2009，
  
  Published：2009
- Accepted：
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Wei, Y., Wang, C., Li, J. et al. Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation., Chin. J. Ocean. Limnol. 27, 558–563 (2009). https://doi.org/10.1007/s00343-009-9166-3
DOI：

Wei, Y., Wang, C., Li, J. et al. Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation., Chin. J. Ocean. Limnol. 27, 558–563 (2009). https://doi.org/10.1007/s00343-009-9166-3 DOI：

摘要

Abstract

We evaluated the effects of high molecular-weight phlorotannins from Sargassum thunbergii (STP) on ADP-induced platelet aggregation and arachidonic acid (AA) metabolism in New Zealand white rabbits and Wistar rats. The inhibition of STP on platelet aggregation was investigated using a turbidimetric method

and the levels of the terminal products of AA metabolism were measured using the corresponding kits for maleic dialdehyde (MDA)

thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) by colorimetry and radioimmunoassay

as appropriate. We found that STP could inhibit ADP-induced platelet aggregation

and the inhibitory ratio was 91.50% at the STP concentration of 4.0 mg/mL. Furthermore

STP markedly affected AA metabolism by decreasing the synthesis of MDA (P<0.01) and increasing the synthesis of 6-keto-PGF1α

thus changing the plasma TXB2/6-keto-PGF1α balance when the platelets were activated (P<0.01). Therefore

STP altered AA metabolism and these findings partly revealed the molecular mechanism by which STP inhibits ADP-induced platelet aggregation.

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references

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