Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides

Jianfeng Niu; Guangce Wang; Fang Lü; Baicheng Zhou; Guang Peng

doi:10.1007/s00343-009-9157-4

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Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides

Updated：2023-05-16

- Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides
- Journal of Oceanology and Limnology Vol. 27, Issue 3, Pages: 502-512(2009)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. College of Marine Science and Engineering Tianjin University of Science and Technology
  3. The Graduate School of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9157-4
  CLC：
- Online First：07 November 2009，
  
  Published：2009
- Accepted：
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Niu, J., Wang, G., Lü, F. et al. Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides, ., Chin. J. Ocean. Limnol. 27, 502–512 (2009). https://doi.org/10.1007/s00343-009-9157-4
DOI：

Niu, J., Wang, G., Lü, F. et al. Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides, ., Chin. J. Ocean. Limnol. 27, 502–512 (2009). https://doi.org/10.1007/s00343-009-9157-4 DOI：

摘要

Abstract

A group of coenocytic marine algae differs from higher plants

whose totipotency depends on an intact cell (or protoplast). Instead

this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals. It is thought that lectins play a key role in the aggregation process. We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides. The lectin was ca. 27 kDa with a pI between pH 5 and pH 6. The absence of carbohydrate suggested that the lectin was not a glycoprotein. The hemagglutinating activity (HA) of the lectin was not dependent on the presence of divalent cations and was inhibited by N-Acetylgalactosamine

N-Acetylglucosamine

and the glycoprotein bovine submaxillary mucin. The lectin preferentially agglutinated Gram-negative bacterium. The HA of this lectin was stable between pH 4 to pH 10. Cell organelles outside the cytoplasm were agglutinated by the addition of lectin solution (0.5 mg ml−1). Our results suggest that the regeneration of B. hypnoides is mediated by this lectin. We also demonstrated that the formation of cell organelle aggregates was inhibited by nigericin in natural seawater (pH 8.0). Given that nigericin dissipates proton gradients across the membrane

we hypothesize that the aggregation of cell organelles was proton-gradient dependent.

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references

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