Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu

Weihong Zhao; Jiangtao Wang; Meimei Chen

doi:10.1007/s00343-009-9141-z

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Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu

Updated：2023-05-16

- Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu
- Journal of Oceanology and Limnology Vol. 27, Issue 3, Pages: 564-569(2009)
- Affiliations：
  
  1. Key Laboratory of Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences
  2. Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9141-z
  CLC：
- Online First：07 November 2009，
  
  Published：2009
- Accepted：
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Zhao, W., Wang, J. & Chen, M. Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu., Chin. J. Ocean. Limnol. 27, 564–569 (2009). https://doi.org/10.1007/s00343-009-9141-z
DOI：

Zhao, W., Wang, J. & Chen, M. Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu., Chin. J. Ocean. Limnol. 27, 564–569 (2009). https://doi.org/10.1007/s00343-009-9141-z DOI：

摘要

Abstract

Filtration and cross-flow ultrafiltration techniques were used to separate culture media of Prorocentrum donghaiense at the exponential growth

stationary and decline stages into <0.45 µm filtrate

100 kDa-0.45 µm

10–100 kDa and 1–10 kDa retentate and <1 kDa ultrafiltrate fractions. The fluorescence properties of different molecular weights of dissolved organic matter (DOM) were measured by excitation-emission matrix spectra. Protein-like and humic-like fluorophores were observed in the DOM produced by P. donghaiense. The central positions of protein-like fluorophores showed a red shift with prolonged growth duration

shifting from tyrosine-like properties at the exponential growth stage to tryptophan-like properties at the stationary and decline stages. The excitation wavelengths of protein-like fluorophores exhibited some change in the exponential growth and stationary stages with increased molecular size

but showed little change in the decline stage. However

the emission wavelengths in the decline stage exhibited a blue shift. Very distinct C type and A type peaks in humic-like fluorophores were observed. With a prolonged culture time

the intensities of both of the peaks became strong and the excitation wavelengths of peak A showed a red shift

while the A:C ratios fell. More than 94% of fluorescent DOM was in the lower than 1 kDa molecular weight fraction.

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