Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses

Feng You; Wei Wang; Dongdong Xu; Xiangping Zhu; Jing Ni; Zhihao Wu; Yongli Xu; Xincheng Wang; Peijun Zhang

doi:10.1007/s00343-009-9134-y

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Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses

Updated：2023-05-16

- Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses
- Journal of Oceanology and Limnology Vol. 27, Issue 2, Pages: 317-323(2009)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Qingdao Agricultural University
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9134-y
  CLC：
- Online First：12 July 2009，
  
  Published：2009
- Accepted：
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You, F., Wang, W., Xu, D. et al. Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses., Chin. J. Ocean. Limnol. 27, 317–323 (2009). https://doi.org/10.1007/s00343-009-9134-y
DOI：

You, F., Wang, W., Xu, D. et al. Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses., Chin. J. Ocean. Limnol. 27, 317–323 (2009). https://doi.org/10.1007/s00343-009-9134-y DOI：

摘要

Abstract

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes

12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them

the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.

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references

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