Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus

Jiye Li; Xiuqin Sun; Fengrong Zheng; Hushan Sun

doi:10.1007/s00343-009-9125-z

Your Location：

Home >

Browse articles >

Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus

Updated：2023-05-16

- Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus
- Journal of Oceanology and Limnology Vol. 27, Issue 3, Pages: 550-554(2009)
- Affiliations：
  
  1. State Oceanic Administration’s North China Sea Environmental Monitoring Center
  2. Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology SOA
  3. The First Institute of Oceanography SOA
  4. College of Life Science Ludong University
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9125-z
  CLC：
- Online First：07 November 2009，
  
  Published：2009
- Accepted：
Scan QR Code
Li, J., Sun, X., Zheng, F. et al. Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus, ., Chin. J. Ocean. Limnol. 27, 550–554 (2009). https://doi.org/10.1007/s00343-009-9125-z
DOI：

Li, J., Sun, X., Zheng, F. et al. Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus, ., Chin. J. Ocean. Limnol. 27, 550–554 (2009). https://doi.org/10.1007/s00343-009-9125-z DOI：

摘要

Abstract

We investigated the distribution of four enzymes involved in the immune response of Apostichopus japonicus. We collected samples of the tentacles

papillate podium

integument

respiratory tree

and digestive tract and stained them for acid phosphatase (ACP)

alkaline phosphatase (AKP)

non-specific esterase (NSE) and peroxidase (POD) activity. The distribution and content of ACP

AKP

NSE

and POD differed among the tissues. The coelomic epithelium of the tentacle

papillate podium

and integument and the mucous layer of respiratory tree were positive for ACP activity. The coelomic epithelium and cuticular layer of the tentacle

papillate podium

and integument and the mucous layer and tunica externa of the respiratory tree and digestive tract stained positive or weakly positive for AKP activity. Almost all the epithelial tissues stained positive

strongly positive

or very strongly positive for NSE activity. The cuticular layer of the tentacle and integument and the mucous layer

tunica submucosa

and tunica externa of the respiratory tree and digestive tract stained positive for POD activity. We hypothesize that these enzymes play a role in the immune response in A. japonicus.

关键词

Keywords

references

Fen C E. 2001. Histochemistry. People’s Medical Publishing House Press, Beijing, China. p. 272–283. (in Chinese)

Liu Z H, Mou H J, Wang Q Y. 2003. Research progress of immune related enzymes in mollusca. Marine Fisheries Research, 24(3): 86–90

Ma Y X, Xu G R, Zhang G R, Wang E P, Chang Y Q. 2006. The etiology of acute preistome edema disease in cultured juveniles of Apostichopus japonicus. Journal of Fishery Sciences of China, 30(3): 377–382

Qiao J H, CHENG B. 2005. The situation and expection of artificial pond culture for Apostichopus joponicus (Selenlca). Marine Sciences, 29(9): 80–82.

Sun S H, Wang Y Y, Han Q, Wu M M, Kao H J. 2006. Histochemistry studies of non-specific esterase and peroxidase on embryo and early larva of oyster Crassostrea gigas. Journal of Fishery Sciences of China, 13(6): 897–901.

Wang P H, Chang Y Q, Xu G R, Song L S. 2005. Isolation and ultrastructure of an enveloped virus in cultured sea cucumber Apostichopus japonicus (Selenka). Journal of Fishery Sciences of China, 12(6): 766–771. (in Chinese with English abstract)

Wang Y Y, Sun S H, Sun X Q, Li G Y. 2004. Peroxidase activity in gill and labial palps of scallop Chlamys farreri. Journal of Fishery Sciences of China, 11(6): 513–518.

Zhang C Y, Wang Y G, Rong X J. 2006. Isolation and identification of causative pathogen for skin ulcerative syndrome in Apostichopus japonicus. Journal of Fishery Sciences of China, 30(1): 118–123. (in Chinese with English abst ract)

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Cyanobacterial extracellular alkaline phosphatase: detection and ecological function

Physiological and molecular responses of invasive cyanobacteriumRaphidiopsis raciborskiito ambient phosphorus deficiency

Screen and effect analysis of immunostimulants for sea cucumber, Apostichopus japonicus

Metabolic rates and biochemical compositions of Apostichopus japonicus (Selenka) tissue during periods of inactivity

The gastric shield and the underlying epithelium ofChlamys farreri: Morphological, histological and histochemical characterizations

Related Author

Lingling WAN

Chunlei SONG

Yiyong ZHOU

Xiuyun CAO

Junqiong SHI

Shuhan HE

Lu ZHAO

Lulu JI

Related Institution

Key Laboratory of Algal Biology, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences

Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Sciences, Southwest University

North China Sea Environmental Monitoring Center State Oceanic Administration

College of Marine Life Science Ocean University of China

Key Laboratory of Mariculture Ministry of Education Ocean University of China

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰