The antitumor effect of bromophenol derivatives in vitro and Leathesia nana extract in vivo

Dayong Shi; Jing Li; Shuju Guo; Hua Su; Xiao Fan

doi:10.1007/s00343-009-9119-x

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The antitumor effect of bromophenol derivatives in vitro and Leathesia nana extract in vivo

Updated：2023-05-16

- The antitumor effect of bromophenol derivatives in vitro and Leathesia nana extract in vivo
- Journal of Oceanology and Limnology Vol. 27, Issue 2, Pages: 277-282(2009)
- Affiliations：
  
  Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9119-x
  CLC：
- Online First：12 July 2009，
  
  Published：2009
- Accepted：
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Shi, D., Li, J., Guo, S. et al. The antitumor effect of bromophenol derivatives in vitro and Leathesia nana extract in vivo, ., Chin. J. Ocean. Limnol. 27, 277–282 (2009). https://doi.org/10.1007/s00343-009-9119-x
DOI：

Shi, D., Li, J., Guo, S. et al. The antitumor effect of bromophenol derivatives in vitro and Leathesia nana extract in vivo, ., Chin. J. Ocean. Limnol. 27, 277–282 (2009). https://doi.org/10.1007/s00343-009-9119-x DOI：

摘要

Abstract

To investigate the antitumor effect of bromophenol derivatives in vitro and Leathesia nana extract in vivo

six bromophenol derivatives 6-(2

3-dibromo-4

5-dihydroxybenzyl)-2

3-dibromo-4

5-dihydroxy benzyl methyl ether (1)

(+)-3-(2

3-dibromo-4

5-dihydroxyphenyl)-4-bromo-5

6-dihydroxy-1

3-dihydroisobenzofuran (2)

3-bromo-4-(2

3-dibromo-4

5-dihydroxybenzyl)-5-methoxymethyl-pyrocatechol (3)

2′

3′-tetrabromo-4

4′

5′-tetrahydroxy-diphenylmethane (4)

bis(2

3-dibromo-4

5-dihydroxybenzyl) ether (5)

2′

3-tribromo-3′

4′

5-tetrahydroxy-6′-ethyloxymethyldiphenylmethane (6) were isolated from brown alga Leathesia nana

and their cytotoxicity were tested by MTT assays in human cancer cell lines A549

BGC-823

MCF-7

B16-BL6

HT-1080

A2780

Bel7402 and HCT-8. Their inhibitory activity against protein tyrosine kinase (PTK) with over-expression of c-kit was analyzed also by ELISA. The antitumor activity of ethanolic extraction of Leathesia nana (EELN) was evaluated on S180-bearing mice. All compounds showed very potent cytotoxicity against all of the eight cancer cell lines with IC50 below 10 μg/mL. In PTK inhibition study

all bromophenol derivatives showed moderate inhibitory activity and compounds 2

5 and 6 showed significant bioactivity with the inhibition ratio of 77.5%

80.1% and 71.4%

respectively. Pharmacological studies reveal that EELN could inhibit the growth of Sarcoma 180 tumor and increase the indices of thymus and spleen to improve the immune system remarkably in vivo. Results indicated that the bromophenol derivatives and EELN can be used as potent antitumor agents for PTK over-expression of c-kit and considered in a new therapeutic strategy for treatment of cancer.

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references

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