Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome

Xiuying Yan; Xiuqin Sun

doi:10.1007/s00343-009-9108-0

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Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome

Updated：2023-05-16

- Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome
- Journal of Oceanology and Limnology Vol. 27, Issue 2, Pages: 337-341(2009)
- Affiliations：
  
  1. College of Llife Science and Technology Ocean University of China
  2. First Institute of Oceanography SOA
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-9108-0
  CLC：
- Online First：12 July 2009，
  
  Published：2009
- Accepted：
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Yan, X., Sun, X. Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome., Chin. J. Ocean. Limnol. 27, 337–341 (2009). https://doi.org/10.1007/s00343-009-9108-0
DOI：

Yan, X., Sun, X. Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome., Chin. J. Ocean. Limnol. 27, 337–341 (2009). https://doi.org/10.1007/s00343-009-9108-0 DOI：

摘要

Abstract

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn)

probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3)

and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene.

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references

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