Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus

Yanjie Chen; Quanqi Zhang; Jie Qi; Yeying Sun; Qiwang Zhong; Xubo Wang; Zhigang Wang; Shuo Li; Chunmei Li

doi:10.1007/s00343-009-0092-1

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Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus

Updated：2023-05-16

- Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus
- Journal of Oceanology and Limnology Vol. 27, Issue 1, Pages: 92-99(2009)
- Affiliations：
  
  Laboratory of Marine Genetics and Breeding Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-0092-1
  CLC：
- Online First：27 March 2009，
  
  Published：2009
- Accepted：
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Chen, Y., Zhang, Q., Qi, J. et al. Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus, ., Chin. J. Ocean. Limnol. 27, 92–99 (2009). https://doi.org/10.1007/s00343-009-0092-1
DOI：

Chen, Y., Zhang, Q., Qi, J. et al. Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus, ., Chin. J. Ocean. Limnol. 27, 92–99 (2009). https://doi.org/10.1007/s00343-009-0092-1 DOI：

摘要

Abstract

Flatfish or flounder moves one eye to change body proportion into vertebral asymmetry during metamorphosis

during which some become sinistral while others dextral. However

the mechanism behinds the eye-position has not been well understood. In this research

hybrids between Japanese flounder(♀) and stone flounder (♂) show mixed eye-location in both dextral type and sinistral type

and thus become good samples for studying the eye-migration. mRNAs from pro-metamorphosis sinistral and dextral hybrids larvae were screened with classical differential display RT-PCR (DD-RT-PCR) and representational difference analysis of cDNA (cDNA-RDA); 30 and 47 putative fragments were isolated

respectively. The cDNA fragments of creatine kinase and trypsinogen 2 precursor genes isolated by cDNA-RDA exhibited eye-position expression patterns during metamorphosis. However

none of the fragments was proved to be related to flatfishes’ eye-position specifically. Therefore

further studies and more sensitive gene isolated methods are needed to solve the problems.

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Keywords

references

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