Initiation of primary cell culture from amphioxus Branchiostoma belcheri tsingtauense

Changliu Wang; Shicui Zhang; Feng Su; Lei Wang; Hongyan Li

doi:10.1007/s00343-009-0069-0

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Initiation of primary cell culture from amphioxus Branchiostoma belcheri tsingtauense

Updated：2023-05-16

- Initiation of primary cell culture from amphioxus Branchiostoma belcheri tsingtauense
- Journal of Oceanology and Limnology Vol. 27, Issue 1, Pages: 69-73(2009)
- Affiliations：
  
  1. School of Life Science Ludong University
  2. Department of Marine Biology Ocean University of China
  3. College of Chemical Engineering Qingdao University of Science and Technology
- Author bio：
- Funds：
- DOI：10.1007/s00343-009-0069-0
  CLC：
- Online First：27 March 2009，
  
  Published：2009
- Accepted：
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Wang, C., Zhang, S., Su, F. et al. Initiation of primary cell culture from amphioxus Branchiostoma belcheri tsingtauense, ., Chin. J. Ocean. Limnol. 27, 69–73 (2009). https://doi.org/10.1007/s00343-009-0069-0
DOI：

Wang, C., Zhang, S., Su, F. et al. Initiation of primary cell culture from amphioxus Branchiostoma belcheri tsingtauense, ., Chin. J. Ocean. Limnol. 27, 69–73 (2009). https://doi.org/10.1007/s00343-009-0069-0 DOI：

摘要

Abstract

Amphioxus

a cephalochordate

is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells

primary cultures were initiated from buccal cirri

tail

gill

gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15

F-12

M 199

MEM

DMEM

PRMI 1640 and LDF

each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C.

关键词

Keywords

references

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